Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02870 and PS417_02875 are separated by 93 nucleotidesPS417_02875 and PS417_02880 are separated by 11 nucleotidesPS417_02880 and PS417_02885 are separated by 47 nucleotidesPS417_02885 and PS417_02890 are separated by 262 nucleotides PS417_02870: PS417_02870 - TonB-dependent receptor, at 656,099 to 658,168 _02870 PS417_02875: PS417_02875 - hypothetical protein, at 658,262 to 658,651 _02875 PS417_02880: PS417_02880 - copper chaperone, at 658,663 to 659,145 _02880 PS417_02885: PS417_02885 - hypothetical protein, at 659,193 to 659,588 _02885 PS417_02890: PS417_02890 - cobalt-precorrin-6X reductase, at 659,851 to 660,570 _02890
Group Condition PS417_02870 PS417_02875 PS417_02880 PS417_02885 PS417_02890
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.1 N.D. -2.8 -0.7 -1.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.3 N.D. -1.7 -3.0 -0.0
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 N.D. -2.1 -1.1 -1.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.1 N.D. -2.8 -1.4 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 N.D. -2.8 -1.3 +0.0
solid stress Scopoletin 2 mM; solid stress -0.9 N.D. -3.6 -0.8 +0.0
temperature LB_noSalt 30C -1.8 N.D. +0.1 -3.1 -0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.6 N.D. -2.0 -1.1 -1.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.7 N.D. -2.1 -1.3 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.8 N.D. -1.8 -1.4 -0.3
stress NaCl stress 25 mM -1.2 N.D. -1.5 -1.3 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 N.D. -2.9 -0.8 +0.0
stress R2A with Polymyxin B sulfate 0.002 mg/ml -1.5 N.D. -1.4 -0.7 -0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.5 N.D. -1.4 -1.4 -0.5
solid stress Scopoletin 3 mM; solid stress -0.7 N.D. -1.5 -1.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.2 N.D. -1.3 -0.6 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 N.D. -3.6 -0.3 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -1.2 N.D. -1.0 -1.0 -0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 N.D. -2.3 -0.5 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.9 N.D. -1.1 -1.0 -0.5
solid stress 80% Methanol 3 mM; solid stress -0.6 N.D. -2.3 -0.6 +0.1
solid stress Fraxetin 2 mM; solid stress -0.3 N.D. -1.1 -2.0 +0.1
solid stress Scopoletin 1 mM; solid stress -0.6 N.D. -2.4 -0.5 +0.4
agar plate interaction control Taped volatile agar plate with no fungus -0.2 N.D. -3.7 +0.5 +0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.2 N.D. -3.2 +0.1 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.9 N.D. +1.9 -2.0 -0.1
solid stress Fraxetin 3 mM; solid stress +0.2 N.D. -2.3 +1.0 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -0.4 N.D. -0.4 -1.4 +1.4
solid stress Fraxetin 3 mM; solid stress +0.4 N.D. -1.4 +1.0 +0.7
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 2500 uM; timepoint 1 -0.5 N.D. +1.5 +1.1 +0.5
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