Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_28420 and PS417_28425 are separated by 150 nucleotidesPS417_28425 and PS417_28430 are separated by 77 nucleotidesPS417_28430 and PS417_28435 overlap by 20 nucleotidesPS417_28435 and PS417_28440 are separated by 36 nucleotides PS417_28420: PS417_28420 - AsnC family transcriptional regulator, at 6,134,464 to 6,134,937 _28420 PS417_28425: PS417_28425 - lysine transporter LysE, at 6,135,088 to 6,135,717 _28425 PS417_28430: PS417_28430 - alpha-2-macroglobulin, at 6,135,795 to 6,141,599 _28430 PS417_28435: PS417_28435 - penicillin-binding protein, at 6,141,580 to 6,143,877 _28435 PS417_28440: PS417_28440 - hypothetical protein, at 6,143,914 to 6,144,279 _28440
Group Condition PS417_28420 PS417_28425 PS417_28430 PS417_28435 PS417_28440
nophagecontrol TIme6-nophage N.D. -0.6 -0.1 -0.1 -0.5
seeds Growth on radish seeds for 72 hours N.D. +0.1 -0.3 -0.1 -1.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.4 -0.1 +0.1 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.8 +0.1 +0.1 -0.6
phage P. simiae OR Antrim MOI 10 N.D. -1.0 +0.1 +0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.2 -0.1 +0.1 -0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. -0.1 -0.0 +0.1 -0.9
soil soil sample 7; outgrowth in LB N.D. -0.4 +0.0 +0.2 -0.7
soil soil sample 5; outgrowth in LB N.D. -0.4 -0.0 +0.3 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.1 +0.1 +0.2 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 -0.1 +0.2 -0.8
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.8 +0.1 +0.0 +0.3
phage P. simiae Grant Run MOI 0.1 N.D. -0.7 +0.0 -0.2 +0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. -0.7 +0.2 +0.3 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 -0.1 -0.3 +0.6
phage P. simiae ORA MOI 1 N.D. -0.6 +0.1 -0.2 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.8 +0.1 +0.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.6 -0.0 +0.3 +0.4
phage P. simiae OR1 MOI 0.1 N.D. +0.2 -0.0 -0.5 +0.5
carbon source L-Valine (C) N.D. -0.4 +0.1 -0.1 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.5 +0.1 +0.2 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.7 +0.0 +0.1 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.8 +0.0 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. -0.4 +0.0 +0.2 +0.9
stress Thallium(I) acetate 0.0625 mg/ml N.D. -0.2 -0.1 -0.1 +1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Methionine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +1.2 -0.1 -0.0 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.2 +0.1 -0.2 +0.9
seeds Growth on radish seeds for 24 hours N.D. +0.8 -0.0 -0.1 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.1 +0.1 +0.3 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Methionine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +1.2 -0.0 -0.0 -0.1
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