Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_28335 and PS417_28340 are separated by 47 nucleotidesPS417_28340 and PS417_28345 are separated by 131 nucleotidesPS417_28345 and PS417_28350 are separated by 5 nucleotidesPS417_28350 and PS417_28355 are separated by 87 nucleotides PS417_28335: PS417_28335 - dihydroorotase, at 6,115,943 to 6,117,280 _28335 PS417_28340: PS417_28340 - hypothetical protein, at 6,117,328 to 6,117,849 _28340 PS417_28345: PS417_28345 - cardiolipin synthetase, at 6,117,981 to 6,119,420 _28345 PS417_28350: PS417_28350 - fatty acid methyltransferase, at 6,119,426 to 6,120,610 _28350 PS417_28355: PS417_28355 - cardiolipin synthetase, at 6,120,698 to 6,121,486 _28355
Group Condition PS417_28335 PS417_28340 PS417_28345 PS417_28350 PS417_28355
soil soil sample 3; outgrowth in LB +0.2 +0.1 -1.1 -1.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 -0.5 +0.0 -1.0 -0.8
soil soil sample 6; outgrowth in LB -0.1 -1.1 +0.1 -0.9 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.6 -0.1 -1.0 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days -0.2 -0.7 +0.3 -0.4 -0.8
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 -0.4 +0.1 -1.1 -0.3
supernatant control Vogels_fungal_media; pH 5.5 +0.0 +0.2 -0.1 -1.3 -0.4
stress Gentamicin 0.008 mg/ml -0.3 -0.3 -0.5 -0.7 +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.2 +0.4 -0.4 -1.0 -0.6
agar plate interaction control Taped volatile agar plate with no fungus +0.0 -0.4 +0.3 -1.3 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +0.3 +0.1 -1.1 -0.5
supernatant control Vogels_fungal_media 0.1X -0.1 -0.5 +0.1 -0.8 +0.5
rhizosphere rhizosphere sample 1; outgrowth in LB -0.1 -0.4 +0.2 -1.0 +0.4
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.1 +0.2 +0.6 -1.6 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.6 -0.1 -0.4 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.3 -0.3 -0.9 +0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +0.1 +0.6 +0.2 -1.2 -0.5
soil soil sample 4; outgrowth in LB +0.2 -0.6 -0.1 -0.9 +0.8
carbon source L-Valine (C) +0.0 -1.2 -0.2 +1.3 -0.2
liquid stress Fraxetin 3 mM; liquid stress +0.2 -0.2 +0.3 -1.0 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.5 -0.5 +0.1 +0.5
soil soil sample 7; outgrowth in LB -0.3 +0.9 +0.1 -0.7 +0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 +0.8 -0.1 -1.1 +0.3
agar plate interaction control Taped volatile agar plate with no fungus -0.0 +0.1 +0.7 -0.9 +0.5
solid stress Fraxetin 2 mM; solid stress +0.2 +0.8 +0.2 -0.5 -0.5
stress methylglyoxal 0.032 vol% -0.0 -0.5 +0.6 +0.8 -0.2
phage Wcs_0.1 +0.1 +1.0 +0.1 +0.3 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +1.0 +0.8 -0.0 -0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 +1.2 +0.1 -1.0 +0.5
carbon source L-Valine (C) +0.3 +0.8 -0.4 +1.2 -0.5
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