Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_28185 and PS417_28190 are separated by 112 nucleotidesPS417_28190 and PS417_28195 overlap by 4 nucleotidesPS417_28195 and PS417_28200 are separated by 3 nucleotidesPS417_28200 and PS417_28205 overlap by 2 nucleotides PS417_28185: PS417_28185 - homocysteine methyltransferase, at 6,085,661 to 6,086,557 _28185 PS417_28190: PS417_28190 - amino acid ABC transporter substrate-binding protein, at 6,086,670 to 6,087,491 _28190 PS417_28195: PS417_28195 - amino acid ABC transporter permease, at 6,087,488 to 6,088,318 _28195 PS417_28200: PS417_28200 - hypothetical protein, at 6,088,322 to 6,088,567 _28200 PS417_28205: PS417_28205 - transporter, at 6,088,566 to 6,089,858 _28205
Group Condition PS417_28185 PS417_28190 PS417_28195 PS417_28200 PS417_28205
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 -0.4 -0.7 -0.5 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.1 -1.0 +0.2 -0.1 -0.4
seeds Growth on radish seeds for 72 hours -0.5 -0.1 -0.4 -1.5 +0.1
stress Gentamicin 0.008 mg/ml -1.7 -0.4 +0.0 +0.6 -0.5
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 -0.7 -0.2 -1.1 -0.0
soil soil sample 7; outgrowth in LB -0.7 -0.7 -0.6 -0.2 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.8 -1.2 +0.0 -0.3 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -1.6 +0.2 -0.1 +0.1
phage P. simiae OR1 MOI 0.1 -0.6 -0.5 +0.0 -1.0 +0.6
carbon source L-Histidine (C) -1.1 -0.5 -0.2 +0.4 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.0 +0.4 -0.0 -0.8 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.4 +0.3 -0.0 -0.8 +0.6
phage P. simiae OR Antrim MOI 10 -0.3 -1.0 +0.0 -0.5 +0.6
soil soil sample 5; outgrowth in LB -1.5 +0.7 -0.3 +0.4 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.3 +0.4 -0.1 +0.3 -0.3
soil soil sample 6; outgrowth in LB -1.0 -0.2 -0.2 +0.6 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X -1.1 +0.2 +0.1 -0.5 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.9 +0.1 +0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant -1.1 +0.7 +0.3 -0.4 +0.1
stress Doxycycline hyclate 0.001 mg/ml -0.8 +0.3 -0.2 +0.7 -0.2
soil soil sample 3; outgrowth in LB +0.3 +0.6 +0.4 -0.4 -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 +0.4 +0.5 -1.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.8 -0.2 +0.0 +1.1 +0.2
seeds Growth on radish seeds for 72 hours +0.0 +0.8 -0.1 -0.8 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 +0.6 +0.4 -0.6 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 -0.6 +0.7 -0.8 +0.5
rhizosphere rhizosphere sample 8; outgrowth in LB +1.0 -0.8 +0.4 +0.2 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.9 -0.1 -0.1 -0.3 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.8 +0.2 +0.3 +0.7 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.4 +0.5 +1.3 +0.3
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