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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_28165 and PS417_28170 are separated by 87 nucleotides
PS417_28170 and PS417_28175 overlap by 4 nucleotides
PS417_28175 and PS417_28180 are separated by 10 nucleotides
PS417_28180 and PS417_28185 are separated by 135 nucleotides
PS417_28165: PS417_28165 - GntR family transcriptional regulator, at 6,082,136 to 6,083,674
_28165
PS417_28170: PS417_28170 - transcriptional regulator, at 6,083,762 to 6,084,400
_28170
PS417_28175: PS417_28175 - GNAT family acetyltransferase, at 6,084,397 to 6,084,843
_28175
PS417_28180: PS417_28180 - GNAT family acetyltransferase, at 6,084,854 to 6,085,525
_28180
PS417_28185: PS417_28185 - homocysteine methyltransferase, at 6,085,661 to 6,086,557
_28185
Group
Condition
PS417
_28165
PS417
_28170
PS417
_28175
PS417
_28180
PS417
_28185
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-1.1
-0.3
-1.0
-0.4
seeds
Growth on radish seeds for 72 hours
-0.2
-0.9
+0.9
-0.7
-1.4
phage
P. simiae OR1 MOI 0.1
+0.1
-0.5
-0.5
-0.9
-0.6
phage
P. simiae Grant Run MOI 10
+0.1
-0.5
-0.8
-0.3
-0.6
soil
soil sample 5; outgrowth in LB
+0.2
-0.4
-0.3
-0.1
-1.5
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.0
-0.4
+0.0
+0.2
-1.5
stress
Gentamicin 0.008 mg/ml
+0.3
-0.3
+0.1
-0.3
-1.7
root
root sample 6; outgrowth in LB
-0.2
-1.3
+1.0
-0.1
-1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
-0.7
-0.5
-0.3
+0.2
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.1
-0.4
-0.6
+0.0
-0.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.2
+0.2
+0.4
-0.8
-1.1
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.6
-0.6
+0.3
+0.0
-0.4
phage
Wcs_0.1
+0.1
-0.6
+0.4
-0.3
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
-1.2
+0.3
+0.4
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-1.1
+0.4
-0.2
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
-1.1
+0.1
+0.4
-0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
+0.4
+0.3
-0.5
-1.0
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
+0.5
-0.4
+0.2
-0.8
motility
inner cut, LB soft agar motility assay
-0.2
-0.3
-0.4
-0.5
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
+0.6
+0.2
-0.2
-1.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.2
+0.1
-0.0
+0.5
-1.3
phage
P. simiae OR Antrim MOI 1
-0.1
-0.9
-0.2
+0.1
+0.6
stress
Doxycycline hyclate 0.001 mg/ml
+0.2
+0.5
-0.5
+0.5
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.5
+0.4
-0.3
+0.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
-0.2
-0.5
-0.2
+1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.8
+0.2
+0.1
+1.3
carbon source
Deoxyribonucleic from herring sperm 5 mg/mL (C)
-0.0
-0.6
+0.3
+0.3
+0.8
carbon source
2-Deoxy-D-ribonic lithium salt 10 mM (C)
-0.0
-0.5
-0.0
+0.5
+1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.1
+0.7
+0.5
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.3
+0.4
+0.3
+1.9
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