Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_28120 and PS417_28125 are separated by 333 nucleotidesPS417_28125 and PS417_28130 are separated by 182 nucleotidesPS417_28130 and PS417_28135 are separated by 392 nucleotidesPS417_28135 and PS417_28140 are separated by 77 nucleotides PS417_28120: PS417_28120 - N5-carboxyaminoimidazole ribonucleotide mutase, at 6,067,917 to 6,068,408 _28120 PS417_28125: PS417_28125 - LysR family transcriptional regulator, at 6,068,742 to 6,069,650 _28125 PS417_28130: PS417_28130 - aspartate ammonia-lyase, at 6,069,833 to 6,071,257 _28130 PS417_28135: PS417_28135 - sodium:alanine symporter, at 6,071,650 to 6,073,095 _28135 PS417_28140: PS417_28140 - asparaginase, at 6,073,173 to 6,074,150 _28140
Group Condition PS417_28120 PS417_28125 PS417_28130 PS417_28135 PS417_28140
no phage control Time6-nophage N.D. N.D. N.D. +0.1 -2.3
phage P. simiae Grant Run MOI 0.1 N.D. N.D. N.D. -0.3 -1.6
phage moi 100 Time6-phageFRS N.D. N.D. N.D. -0.1 -1.6
soil soil sample 7; outgrowth in LB N.D. N.D. N.D. +0.1 -1.6
nophagecontrol TIme6-nophage N.D. N.D. N.D. +0.1 -1.6
root root sample 6; outgrowth in LB N.D. N.D. N.D. -0.3 -1.0
phage P. simiae Grant Run MOI 0.1 N.D. N.D. N.D. -0.2 -1.1
phage P. simiae OR Antrim MOI 10 N.D. N.D. N.D. -0.4 -0.8
phage JP1 MOI 0.1 N.D. N.D. N.D. -0.3 -0.8
nophagecontrol TIme6-nophage N.D. N.D. N.D. +0.2 -1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.2 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.0 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.2 -0.9
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.1 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.1 -1.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.2 -1.1
stress methylglyoxal 0.032 vol% N.D. N.D. N.D. +0.2 -0.9
soil soil sample 6; outgrowth in LB N.D. N.D. N.D. +0.1 -0.9
soil soil sample 4; outgrowth in LB N.D. N.D. N.D. +0.3 -1.0
carbon source L-Asparagine (C) N.D. N.D. N.D. +0.8 -0.5
carbon source L-Asparagine (C) N.D. N.D. N.D. +0.8 -0.3
carbon source L-Glutamine (C) N.D. N.D. N.D. +1.1 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.5 +0.6
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.1 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.3 +0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.3 +1.2
carbon source L-Valine (C) N.D. N.D. N.D. +1.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.4 +1.5
phage P. simiae OR1 MOI 0.1 N.D. N.D. N.D. -0.1 +2.4
carbon source L-Valine (C) N.D. N.D. N.D. +1.9 +0.5
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