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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_28110 and PS417_28115 are separated by 104 nucleotides
PS417_28115 and PS417_28120 are separated by 11 nucleotides
PS417_28120 and PS417_28125 are separated by 333 nucleotides
PS417_28125 and PS417_28130 are separated by 182 nucleotides
PS417_28110: PS417_28110 - transglycosylase, at 6,066,470 to 6,066,715
_28110
PS417_28115: PS417_28115 - phosphoribosylaminoimidazole carboxylase, at 6,066,820 to 6,067,905
_28115
PS417_28120: PS417_28120 - N5-carboxyaminoimidazole ribonucleotide mutase, at 6,067,917 to 6,068,408
_28120
PS417_28125: PS417_28125 - LysR family transcriptional regulator, at 6,068,742 to 6,069,650
_28125
PS417_28130: PS417_28130 - aspartate ammonia-lyase, at 6,069,833 to 6,071,257
_28130
Group
Condition
PS417
_28110
PS417
_28115
PS417
_28120
PS417
_28125
PS417
_28130
phage
JP1 MOI 1
-1.0
N.D.
N.D.
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
-1.0
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
N.D.
N.D.
N.D.
N.D.
seeds
Growth on radish seeds for 24 hours
-0.8
N.D.
N.D.
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
-0.8
N.D.
N.D.
N.D.
N.D.
phage
P. simiae ORA MOI 0.1
-0.8
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.7
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.7
N.D.
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.6
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
N.D.
N.D.
N.D.
N.D.
phage
FRSphage
-0.6
N.D.
N.D.
N.D.
N.D.
phage
JP1 MOI 0.1
-0.6
N.D.
N.D.
N.D.
N.D.
stress
methylglyoxal 0.032 vol%
-0.6
N.D.
N.D.
N.D.
N.D.
carbon source
Fumarate (C)
-0.6
N.D.
N.D.
N.D.
N.D.
solid stress
Fraxetin 3 mM; solid stress
-0.6
N.D.
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 1X
-0.6
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
N.D.
N.D.
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
N.D.
N.D.
N.D.
N.D.
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
+0.6
N.D.
N.D.
N.D.
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.6
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
N.D.
N.D.
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.8
N.D.
N.D.
N.D.
N.D.
soil
soil sample 6; outgrowth in LB
+0.8
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.8
N.D.
N.D.
N.D.
N.D.
soil
soil sample 2; outgrowth in LB
+0.9
N.D.
N.D.
N.D.
N.D.
soil
soil sample 4; outgrowth in LB
+1.1
N.D.
N.D.
N.D.
N.D.
root
root sample 6; outgrowth in LB
+1.4
N.D.
N.D.
N.D.
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
+1.7
N.D.
N.D.
N.D.
N.D.
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