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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_28010 and PS417_28015 are separated by 37 nucleotides
PS417_28015 and PS417_28020 are separated by 196 nucleotides
PS417_28020 and PS417_28025 overlap by 1 nucleotides
PS417_28025 and PS417_28030 are separated by 111 nucleotides
PS417_28010: PS417_28010 - pyridine nucleotide-disulfide oxidoreductase, at 6,046,340 to 6,047,488
_28010
PS417_28015: PS417_28015 - rubredoxin, at 6,047,526 to 6,047,693
_28015
PS417_28020: PS417_28020 - chorismate--pyruvate lyase, at 6,047,890 to 6,048,453
_28020
PS417_28025: PS417_28025 - 4-hydroxybenzoate polyprenyltransferase, at 6,048,453 to 6,049,343
_28025
PS417_28030: PS417_28030 - hypothetical protein, at 6,049,455 to 6,049,832
_28030
Group
Condition
PS417
_28010
PS417
_28015
PS417
_28020
PS417
_28025
PS417
_28030
phage
P. simiae OR Antrim MOI 10
-0.6
-0.4
-1.4
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.6
-0.8
N.D.
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.3
-0.1
-1.7
N.D.
-0.5
rhizosphere
rhizosphere sample 2; outgrowth in LB
-2.1
-0.6
+0.0
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.1
-1.4
N.D.
-1.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
+0.0
-1.6
N.D.
-0.8
phage moi 100
Time6-phageFRS
+0.2
-0.2
-1.6
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.0
-1.5
N.D.
-0.6
liquid stress
Scopoletin 3 mM; liquid stress
-0.3
-0.8
-1.1
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.1
+0.3
N.D.
-1.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.7
-0.2
-1.5
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-0.3
-1.1
N.D.
-0.2
no phage control
Time6-nophage
+0.2
+0.6
-0.8
N.D.
-0.9
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.5
-1.0
N.D.
+0.4
seeds
Growth on radish seeds for 72 hours
+0.5
+0.3
+0.5
N.D.
-1.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.5
-1.3
N.D.
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.9
-0.2
-0.6
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
-0.2
+0.6
N.D.
+0.4
carbon source
Carbon source D-Mannitol 2.5 mM
+0.5
-0.4
-0.9
N.D.
+0.4
seeds
Growth on radish seeds for 72 hours
-0.5
+1.1
+0.3
N.D.
-1.1
root
root sample 6; outgrowth in LB
+0.1
-0.8
+0.8
N.D.
+0.8
phage
JP1 MOI 1
+0.1
+0.8
+1.0
N.D.
-0.8
seeds
Growth on radish seeds for 72 hours
+0.4
+1.1
+0.5
N.D.
-0.8
motility
inner cut, LB soft agar motility assay
+0.4
+0.3
+1.0
N.D.
-0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+1.4
+0.5
-0.8
N.D.
+0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
+1.3
-0.2
N.D.
+0.6
stress
Doxycycline hyclate 0.001 mg/ml
+0.9
+0.8
+0.3
N.D.
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
+0.0
-0.3
+1.2
N.D.
+0.7
stress
Phosphomycin 0.001 mg/ml
+1.8
-0.8
+1.0
N.D.
+0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+1.2
+0.6
+0.2
N.D.
+0.9
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