Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_28010 and PS417_28015 are separated by 37 nucleotidesPS417_28015 and PS417_28020 are separated by 196 nucleotidesPS417_28020 and PS417_28025 overlap by 1 nucleotidesPS417_28025 and PS417_28030 are separated by 111 nucleotides PS417_28010: PS417_28010 - pyridine nucleotide-disulfide oxidoreductase, at 6,046,340 to 6,047,488 _28010 PS417_28015: PS417_28015 - rubredoxin, at 6,047,526 to 6,047,693 _28015 PS417_28020: PS417_28020 - chorismate--pyruvate lyase, at 6,047,890 to 6,048,453 _28020 PS417_28025: PS417_28025 - 4-hydroxybenzoate polyprenyltransferase, at 6,048,453 to 6,049,343 _28025 PS417_28030: PS417_28030 - hypothetical protein, at 6,049,455 to 6,049,832 _28030
Group Condition PS417_28010 PS417_28015 PS417_28020 PS417_28025 PS417_28030
phage P. simiae OR Antrim MOI 10 -0.6 -0.4 -1.4 N.D. -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.6 -0.8 N.D. -0.7
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.3 -0.1 -1.7 N.D. -0.5
rhizosphere rhizosphere sample 2; outgrowth in LB -2.1 -0.6 +0.0 N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.1 -1.4 N.D. -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.0 +0.0 -1.6 N.D. -0.8
phage moi 100 Time6-phageFRS +0.2 -0.2 -1.6 N.D. -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.0 -1.5 N.D. -0.6
liquid stress Scopoletin 3 mM; liquid stress -0.3 -0.8 -1.1 N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.1 +0.3 N.D. -1.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 -0.2 -1.5 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -0.3 -1.1 N.D. -0.2
no phage control Time6-nophage +0.2 +0.6 -0.8 N.D. -0.9
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 +0.5 -1.0 N.D. +0.4
seeds Growth on radish seeds for 72 hours +0.5 +0.3 +0.5 N.D. -1.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.5 -1.3 N.D. -0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.9 -0.2 -0.6 N.D. -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 -0.2 +0.6 N.D. +0.4
carbon source Carbon source D-Mannitol 2.5 mM +0.5 -0.4 -0.9 N.D. +0.4
seeds Growth on radish seeds for 72 hours -0.5 +1.1 +0.3 N.D. -1.1
root root sample 6; outgrowth in LB +0.1 -0.8 +0.8 N.D. +0.8
phage JP1 MOI 1 +0.1 +0.8 +1.0 N.D. -0.8
seeds Growth on radish seeds for 72 hours +0.4 +1.1 +0.5 N.D. -0.8
motility inner cut, LB soft agar motility assay +0.4 +0.3 +1.0 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +1.4 +0.5 -0.8 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +1.3 -0.2 N.D. +0.6
stress Doxycycline hyclate 0.001 mg/ml +0.9 +0.8 +0.3 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 -0.3 +1.2 N.D. +0.7
stress Phosphomycin 0.001 mg/ml +1.8 -0.8 +1.0 N.D. +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.2 +0.6 +0.2 N.D. +0.9
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