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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_02775 and PS417_02780 are separated by 238 nucleotides
PS417_02780 and PS417_02785 are separated by 73 nucleotides
PS417_02785 and PS417_02790 are separated by 113 nucleotides
PS417_02790 and PS417_02795 overlap by 4 nucleotides
PS417_02775: PS417_02775 - heat-shock protein, at 630,748 to 632,016
_02775
PS417_02780: PS417_02780 - DNA-binding protein, at 632,255 to 633,199
_02780
PS417_02785: PS417_02785 - Urease (EC 3.5.1.5)
(from data)
, at 633,273 to 634,973
_02785
PS417_02790: PS417_02790 - urease subunit beta, at 635,087 to 635,392
_02790
PS417_02795: PS417_02795 - acetyltransferase, at 635,389 to 635,922
_02795
Group
Condition
PS417
_02775
PS417
_02780
PS417
_02785
PS417
_02790
PS417
_02795
nitrogen source
Urea (N)
-0.2
-0.1
-4.5
N.D.
-1.4
nitrogen source
Inosine (N)
-0.0
-0.2
-4.2
N.D.
-1.5
motility
inner cut, LB soft agar motility assay
-0.8
-0.8
-0.2
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.7
-1.2
-0.3
N.D.
+0.1
phage
P. simiae OR1 MOI 0.1
-1.2
-0.0
-0.1
N.D.
-0.7
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.6
-0.7
-0.1
N.D.
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
-0.4
-0.6
-0.3
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.2
-0.8
N.D.
-0.3
solid stress
80% Methanol 2 mM; solid stress
-0.7
-0.5
-0.3
N.D.
-0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
-0.3
-0.4
N.D.
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.8
-0.6
-0.6
N.D.
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
-0.8
-0.0
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
+0.3
-0.4
N.D.
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.4
-0.5
N.D.
+0.1
solid stress
Fraxetin 3 mM; solid stress
-0.4
-0.7
-0.2
N.D.
+0.1
carbon source
L-Valine (C)
-0.3
-0.7
-0.4
N.D.
+0.2
phage
P. simiae OR Antrim MOI 0.1
-0.8
+0.2
-0.1
N.D.
-0.4
solid stress
80% Methanol 1 mM; solid stress
-0.1
-1.1
+0.1
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.2
-1.0
+0.1
N.D.
-0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.7
-0.4
+0.2
N.D.
+0.1
soil
soil sample 5; outgrowth in LB
+0.4
-0.1
-0.0
N.D.
-0.9
seeds
Growth on radish seeds for 72 hours
-1.0
+0.1
-0.2
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.6
+0.4
-0.0
N.D.
-0.4
soil
soil sample 2; outgrowth in LB
-0.2
+0.7
-0.4
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-0.4
+0.3
N.D.
+0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
-0.5
+0.9
N.D.
+0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.4
+0.3
+0.8
N.D.
-0.2
phage
JP1 MOI 10
+0.8
+0.6
+0.1
N.D.
-0.7
stress
methylglyoxal 0.032 vol%
+0.4
-0.3
+0.2
N.D.
+0.6
stress
Gentamicin 0.008 mg/ml
+1.3
+0.4
-0.1
N.D.
+0.5
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