Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27955 and PS417_27960 are separated by 172 nucleotidesPS417_27960 and PS417_27965 are separated by 90 nucleotidesPS417_27965 and PS417_27970 overlap by 4 nucleotidesPS417_27970 and PS417_27975 overlap by 37 nucleotides PS417_27955: PS417_27955 - EvpB family type VI secretion protein, at 6,030,029 to 6,031,525 _27955 PS417_27960: PS417_27960 - Hcp1 family type VI secretion system effector, at 6,031,698 to 6,032,186 _27960 PS417_27965: PS417_27965 - hypothetical protein, at 6,032,277 to 6,032,792 _27965 PS417_27970: PS417_27970 - type VI secretion system protein ImpG, at 6,032,789 to 6,034,648 _27970 PS417_27975: PS417_27975 - type VI secretion protein, at 6,034,612 to 6,035,655 _27975
Group Condition PS417_27955 PS417_27960 PS417_27965 PS417_27970 PS417_27975
phage JP1 MOI 10 -0.2 -0.6 -1.1 +0.1 +0.0
stress Gentamicin 0.008 mg/ml -0.3 -0.1 -0.4 -0.1 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.8 -0.0 +0.1 -0.6
soil soil sample 3; outgrowth in LB -0.5 +0.0 -0.3 -0.1 -0.7
soil soil sample 4; outgrowth in LB -0.3 -0.2 -0.6 +0.1 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.4 +0.3 -0.2 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 -0.9 -0.2 -0.1 -0.3
phage P. simiae OR Antrim MOI 10 -0.2 +0.3 -0.4 -0.4 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.5 +0.3 -0.3 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 +0.3 -0.2 +0.2 -0.6
agar plate interaction control Taped volatile agar plate with no fungus +0.2 -1.1 +0.4 +0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Threonine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -0.7 +0.4 +0.3 -0.4
phage JP1 MOI 1 +0.0 -0.5 -0.3 -0.4 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.1 +0.2 +0.6 -1.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.6 +0.7 -0.0 -0.2
rhizosphere rhizosphere sample 2; outgrowth in LB -0.2 -0.2 +0.6 +0.2 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -0.8 +0.2 -0.0 +0.3
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.1 -0.3 +0.3 +0.5 -0.3
rhizosphere rhizosphere sample 8; outgrowth in LB +0.1 -0.7 +0.7 +0.3 -0.3
phage P. simiae OR Antrim MOI 1 +0.3 +0.5 -0.1 +0.2 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.1 -0.4 +1.1 -0.0 -0.5
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 +0.3 -0.5 +0.6 +0.1
seeds Growth on radish seeds for 72 hours -0.4 -0.1 +0.3 -0.3 +0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +0.9 +0.4 +0.3 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.2 +0.5 +0.8 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.0 -0.2 +0.9 -0.2
root root sample 6; outgrowth in LB +0.2 -0.4 -0.3 +0.2 +1.1
carbon source L-Valine (C) +0.1 +0.6 +0.7 -0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 +0.1 -0.2 +0.9 +0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.5 -0.1 +0.4 +0.7
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