Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27755 and PS417_27760 are separated by 11 nucleotidesPS417_27760 and PS417_27765 are separated by 458 nucleotidesPS417_27765 and PS417_27770 are separated by 27 nucleotidesPS417_27770 and PS417_27775 are separated by 141 nucleotides PS417_27755: PS417_27755 - 50S ribosomal protein L33, at 5,986,477 to 5,986,632 _27755 PS417_27760: PS417_27760 - 50S ribosomal protein L28, at 5,986,644 to 5,986,877 _27760 PS417_27765: PS417_27765 - peptide ABC transporter substrate-binding protein, at 5,987,336 to 5,988,928 _27765 PS417_27770: PS417_27770 - hypothetical protein, at 5,988,956 to 5,989,630 _27770 PS417_27775: PS417_27775 - phosphopantothenoylcysteine decarboxylase, at 5,989,772 to 5,990,980 _27775
Group Condition PS417_27755 PS417_27760 PS417_27765 PS417_27770 PS417_27775
rhizosphere rhizosphere sample 2; outgrowth in LB N.D. N.D. -0.3 -1.3 N.D.
carbon source L-Valine (C) N.D. N.D. -0.8 -0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.5 -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.5 -0.7 N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. -0.0 -1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. N.D. -0.4 -0.6 N.D.
carbon source Putrescine (C) N.D. N.D. -0.4 -0.7 N.D.
agar plate interaction control Taped volatile agar plate with no fungus N.D. N.D. -0.1 -0.9 N.D.
supernatant control:fungal media R2A with Vogels_fungal_media 0.8X N.D. N.D. +0.2 -1.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.1 -1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB N.D. N.D. -0.1 -0.9 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.1 -0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.2 -0.8 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.5 -0.4 N.D.
solid stress Scopoletin 1 mM; solid stress N.D. N.D. -0.3 -0.6 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -0.2 -0.7 N.D.
seeds Growth on radish seeds for 72 hours N.D. N.D. -0.0 -0.8 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.3 -1.0 N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB N.D. N.D. +0.2 -0.7 N.D.
soil soil sample 6; outgrowth in LB N.D. N.D. +0.3 -0.7 N.D.
phage Wcs_0.1 N.D. N.D. -0.3 +0.6 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.8 -0.4 N.D.
carbon source L-Valine (C) N.D. N.D. -0.2 +0.8 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. +0.6 +0.3 N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. +0.3 +0.6 N.D.
stress Neomycin 0.04 mg/ml N.D. N.D. +1.1 +0.0 N.D.
seeds Growth on radish seeds for 24 hours N.D. N.D. +0.4 +0.8 N.D.
stress Gentamicin 0.008 mg/ml N.D. N.D. +0.8 +0.3 N.D.
phage P. simiae OR Antrim MOI 1 N.D. N.D. +0.6 +0.8 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. -0.1 +1.6 N.D.
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