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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_27665 and PS417_27670 are separated by 255 nucleotides
PS417_27670 and PS417_27675 are separated by 58 nucleotides
PS417_27675 and PS417_27680 are separated by 134 nucleotides
PS417_27680 and PS417_27685 are separated by 133 nucleotides
PS417_27665: PS417_27665 - diguanylate cyclase, at 5,966,268 to 5,967,932
_27665
PS417_27670: PS417_27670 - ATP-dependent DNA helicase Rep, at 5,968,188 to 5,970,197
_27670
PS417_27675: PS417_27675 - xanthine phosphoribosyltransferase, at 5,970,256 to 5,970,828
_27675
PS417_27680: PS417_27680 - acetyl-CoA hydrolase, at 5,970,963 to 5,972,885
_27680
PS417_27685: PS417_27685 - cytochrome C, at 5,973,019 to 5,973,444
_27685
Group
Condition
PS417
_27665
PS417
_27670
PS417
_27675
PS417
_27680
PS417
_27685
soil
soil sample 3; outgrowth in LB
-0.3
N.D.
-2.6
-0.0
N.D.
stress
methylglyoxal 0.032 vol%
-0.9
N.D.
-1.8
+0.5
N.D.
motility
inner cut, LB soft agar motility assay
-1.1
N.D.
-0.6
-0.2
N.D.
phage
JP1 MOI 10
-1.3
N.D.
-0.1
-0.3
N.D.
phage
P. simiae Grant Run MOI 0.1
-0.6
N.D.
-0.7
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
N.D.
-1.2
+0.3
N.D.
nitrogen source
Uridine (N)
-1.4
N.D.
+0.1
+0.1
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
-0.7
N.D.
-0.9
+0.5
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
-1.5
+0.6
N.D.
phage
JP1 MOI 0.1
-1.2
N.D.
-0.0
+0.5
N.D.
soil
soil sample 2; outgrowth in LB
+0.5
N.D.
-1.2
-0.0
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
N.D.
-1.2
+0.5
N.D.
carbon source
2'-Deoxyinosine 5 mM (C)
-0.9
N.D.
+0.6
-0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.6
N.D.
-0.7
+0.8
N.D.
soil
soil sample 5; outgrowth in LB
+0.9
N.D.
-0.9
-0.4
N.D.
supernatant control
Vogels_fungal_media 0.1X
-1.2
N.D.
+0.5
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
N.D.
+0.9
+0.3
N.D.
stress
6-methoxy-2(3H)-benzoxazolone 2.5 mM
+1.1
N.D.
-0.9
-0.1
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
N.D.
+0.8
+0.3
N.D.
rhizosphere
rhizosphere sample 2; outgrowth in LB
+1.0
N.D.
-0.2
+0.5
N.D.
stress
6-methoxy-2(3H)-benzoxazolone 0.5 mM
+1.2
N.D.
+0.2
-0.2
N.D.
root
root sample 6; outgrowth in LB
+1.4
N.D.
-0.2
+0.4
N.D.
soil
soil sample 8; outgrowth in LB
+0.4
N.D.
+0.8
+0.6
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+1.0
N.D.
+0.6
+0.2
N.D.
carbon source
2-Deoxy-D-Ribose 10 mM (C)
+0.4
N.D.
+1.0
+0.4
N.D.
rhizosphere
rhizosphere sample 8; outgrowth in LB
+1.6
N.D.
+0.2
+0.1
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+1.9
N.D.
-0.1
+0.2
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+2.3
N.D.
+0.0
+0.9
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+3.2
N.D.
-0.0
+0.1
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+3.4
N.D.
+0.6
-0.4
N.D.
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