Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27365 and PS417_27370 are separated by 156 nucleotidesPS417_27370 and PS417_27375 are separated by 235 nucleotidesPS417_27375 and PS417_27380 are separated by 121 nucleotidesPS417_27380 and PS417_27385 are separated by 255 nucleotides PS417_27365: PS417_27365 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 5,904,491 to 5,905,957 _27365 PS417_27370: PS417_27370 - transcription termination factor Rho, at 5,906,114 to 5,907,373 _27370 PS417_27375: PS417_27375 - thioredoxin, at 5,907,609 to 5,907,938 _27375 PS417_27380: PS417_27380 - GntR family transcriptional regulator, at 5,908,060 to 5,908,755 _27380 PS417_27385: PS417_27385 - amino acid ABC transporter substrate-binding protein, at 5,909,011 to 5,909,796 _27385
Group Condition PS417_27365 PS417_27370 PS417_27375 PS417_27380 PS417_27385
phage P. simiae OR1 MOI 0.1 N.D. N.D. N.D. -0.7 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -1.2 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.6 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. -0.9 -0.1
nophagecontrol Only library N.D. N.D. N.D. -0.8 -0.3
agar plate interaction control Taped volatile agar plate with no fungus N.D. N.D. N.D. -0.9 -0.2
soil soil sample 6; outgrowth in LB N.D. N.D. N.D. -0.8 -0.2
root root sample 6; outgrowth in LB N.D. N.D. N.D. -0.9 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -1.0 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.6 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -1.1 +0.3
nophagecontrol TIme6-nophage N.D. N.D. N.D. -0.7 +0.2
motility outer cut, LB soft agar motility assay N.D. N.D. N.D. -0.7 +0.2
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.7 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.5 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.5 +0.7
carbon source L-Valine (C) N.D. N.D. N.D. -0.5 +0.7
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. N.D. N.D. +0.6 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.9 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. N.D. +0.3 +0.6
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.8 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.6 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.5 +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.8 +0.1
carbon source L-Leucine (C) N.D. N.D. N.D. +0.6 +0.3
phage P. simiae OR Antrim MOI 0.1 N.D. N.D. N.D. +0.7 +0.2
phage P. simiae OR Antrim MOI 1 N.D. N.D. N.D. +0.8 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.7 +0.3
motility inner cut, LB soft agar motility assay N.D. N.D. N.D. +1.2 +0.0
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