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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_27330 and PS417_27335 are separated by 52 nucleotides
PS417_27335 and PS417_27340 are separated by 150 nucleotides
PS417_27340 and PS417_27345 are separated by 292 nucleotides
PS417_27345 and PS417_27350 overlap by 4 nucleotides
PS417_27330: PS417_27330 - membrane protein, at 5,897,611 to 5,898,726
_27330
PS417_27335: PS417_27335 - flagellar basal body protein FliL, at 5,898,779 to 5,899,186
_27335
PS417_27340: PS417_27340 - NADPH:quinone oxidoreductase, at 5,899,337 to 5,900,314
_27340
PS417_27345: PS417_27345 - sn-glycerol-3-phosphate transporter, at 5,900,607 to 5,901,944
_27345
PS417_27350: PS417_27350 - calcium transporter ChaC, at 5,901,941 to 5,902,594
_27350
Group
Condition
PS417
_27330
PS417
_27335
PS417
_27340
PS417
_27345
PS417
_27350
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
-1.1
-0.6
+0.0
-0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
+0.3
-0.9
-0.0
-1.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
+0.5
-2.2
-0.1
+0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
+0.3
-1.8
+0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-1.0
-0.8
+0.5
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
-1.3
-0.2
+0.2
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-1.2
+0.2
+0.0
+0.5
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.3
+0.5
-1.1
+0.2
-0.1
phage
JP1 MOI 10
-0.1
+0.8
-0.9
+0.2
-0.1
phage
JP1 MOI 10
-0.3
+1.3
-0.8
+0.0
-0.2
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB
+0.3
+0.5
-1.0
+0.1
+0.4
phage
JP1 MOI 1
+0.1
+1.1
+0.3
-0.2
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.8
+1.0
+0.2
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
-1.4
+0.8
+0.2
+0.5
agar plate interaction control
Taped volatile agar plate with no fungus
+0.3
+0.0
-0.6
-0.0
+1.2
soil
soil sample 4; outgrowth in LB
-0.1
+0.9
-0.7
+0.3
+0.4
soil
soil sample 7; outgrowth in LB
+0.2
+0.8
-0.6
+0.5
+0.0
nophagecontrol
Only library
+0.0
+1.1
+0.6
-0.2
-0.6
soil
soil sample 2; outgrowth in LB
+0.4
+0.7
-0.6
+0.4
+0.3
phage
JP1 MOI 0.1
-0.1
+0.9
+1.0
+0.1
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.5
+0.7
-1.1
+0.2
-0.0
stress
1-ethyl-3-methylimidazolium chloride 200 mM
-0.2
+1.3
+0.5
+0.2
-0.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+1.2
+0.3
+0.1
+0.4
-0.4
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+1.2
-0.1
-0.1
+0.1
+0.7
stress
Choline chloride 300 mM
-0.0
+1.5
+0.6
+0.0
-0.2
rhizosphere
rhizosphere sample 8; outgrowth in LB
+0.3
+1.1
+0.9
+0.1
+0.0
stress
Bacitracin 1 mg/ml
+0.2
+0.3
+0.2
+2.5
+0.2
stress
Phosphomycin 0.002 mg/ml
+0.4
+1.1
+1.5
+9.1
+1.1
stress
Phosphomycin 0.001 mg/ml
+0.3
+1.0
+1.2
+10.0
+0.8
stress
Phosphomycin 0.003 mg/ml
+0.4
+1.4
+1.6
+9.4
+1.2
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