Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27330 and PS417_27335 are separated by 52 nucleotidesPS417_27335 and PS417_27340 are separated by 150 nucleotidesPS417_27340 and PS417_27345 are separated by 292 nucleotidesPS417_27345 and PS417_27350 overlap by 4 nucleotides PS417_27330: PS417_27330 - membrane protein, at 5,897,611 to 5,898,726 _27330 PS417_27335: PS417_27335 - flagellar basal body protein FliL, at 5,898,779 to 5,899,186 _27335 PS417_27340: PS417_27340 - NADPH:quinone oxidoreductase, at 5,899,337 to 5,900,314 _27340 PS417_27345: PS417_27345 - sn-glycerol-3-phosphate transporter, at 5,900,607 to 5,901,944 _27345 PS417_27350: PS417_27350 - calcium transporter ChaC, at 5,901,941 to 5,902,594 _27350
Group Condition PS417_27330 PS417_27335 PS417_27340 PS417_27345 PS417_27350
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -1.1 -0.6 +0.0 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.3 -0.9 -0.0 -1.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.0 +0.5 -2.2 -0.1 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 +0.3 -1.8 +0.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -1.0 -0.8 +0.5 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 -1.3 -0.2 +0.2 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -1.2 +0.2 +0.0 +0.5
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 +0.5 -1.1 +0.2 -0.1
phage JP1 MOI 10 -0.1 +0.8 -0.9 +0.2 -0.1
phage JP1 MOI 10 -0.3 +1.3 -0.8 +0.0 -0.2
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB +0.3 +0.5 -1.0 +0.1 +0.4
phage JP1 MOI 1 +0.1 +1.1 +0.3 -0.2 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 -0.8 +1.0 +0.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.6 -1.4 +0.8 +0.2 +0.5
agar plate interaction control Taped volatile agar plate with no fungus +0.3 +0.0 -0.6 -0.0 +1.2
soil soil sample 4; outgrowth in LB -0.1 +0.9 -0.7 +0.3 +0.4
soil soil sample 7; outgrowth in LB +0.2 +0.8 -0.6 +0.5 +0.0
nophagecontrol Only library +0.0 +1.1 +0.6 -0.2 -0.6
soil soil sample 2; outgrowth in LB +0.4 +0.7 -0.6 +0.4 +0.3
phage JP1 MOI 0.1 -0.1 +0.9 +1.0 +0.1 -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 +0.7 -1.1 +0.2 -0.0
stress 1-ethyl-3-methylimidazolium chloride 200 mM -0.2 +1.3 +0.5 +0.2 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.2 +0.3 +0.1 +0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.2 -0.1 -0.1 +0.1 +0.7
stress Choline chloride 300 mM -0.0 +1.5 +0.6 +0.0 -0.2
rhizosphere rhizosphere sample 8; outgrowth in LB +0.3 +1.1 +0.9 +0.1 +0.0
stress Bacitracin 1 mg/ml +0.2 +0.3 +0.2 +2.5 +0.2
stress Phosphomycin 0.002 mg/ml +0.4 +1.1 +1.5 +9.1 +1.1
stress Phosphomycin 0.001 mg/ml +0.3 +1.0 +1.2 +10.0 +0.8
stress Phosphomycin 0.003 mg/ml +0.4 +1.4 +1.6 +9.4 +1.2
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