Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27270 and PS417_27275 are separated by 3 nucleotidesPS417_27275 and PS417_27280 overlap by 8 nucleotidesPS417_27280 and PS417_27285 overlap by 4 nucleotidesPS417_27285 and PS417_27290 are separated by 19 nucleotides PS417_27270: PS417_27270 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 5,886,714 to 5,887,937 _27270 PS417_27275: PS417_27275 - hypothetical protein, at 5,887,941 to 5,888,426 _27275 PS417_27280: PS417_27280 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 5,888,419 to 5,889,606 _27280 PS417_27285: PS417_27285 - Xaa-Pro aminopeptidase, at 5,889,603 to 5,890,925 _27285 PS417_27290: PS417_27290 - hypothetical protein, at 5,890,945 to 5,891,502 _27290
Group Condition PS417_27270 PS417_27275 PS417_27280 PS417_27285 PS417_27290
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.3 N.D. +0.8 -5.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.1 N.D. -0.5 -5.2
solid stress Fraxetin 2 mM; solid stress N.D. +0.1 N.D. +0.5 -5.4
solid stress Fraxetin 3 mM; solid stress N.D. +0.0 N.D. +1.0 -5.2
supernatant control:fungal media R2A with Vogels_fungal_media 0.2X N.D. -0.3 N.D. +0.1 -3.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. -0.2 N.D. -0.5 -2.8
carbon source L-Valine (C) N.D. +0.2 N.D. +0.2 -3.7
stress Polymyxin B sulfate 0.006 mg/ml N.D. +0.1 N.D. -3.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.7 N.D. -0.5 -1.8
phage JP1 MOI 10 N.D. +0.2 N.D. +0.0 -2.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 N.D. -0.6 -1.5
phage JP1 MOI 1 N.D. +0.2 N.D. +0.4 -3.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.4 N.D. -0.1 -1.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.4 N.D. -0.2 -1.6
phage JP1 MOI 10 N.D. -0.0 N.D. -0.0 -2.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.4 N.D. -0.5 -2.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -1.1 N.D. +0.3 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.4 N.D. -0.7 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 N.D. -0.5 -1.5
phage P. simiae OR Antrim MOI 1 N.D. +0.3 N.D. +0.1 -2.1
seeds Growth on radish seeds for 72 hours N.D. +0.4 N.D. -0.3 -1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -1.1 N.D. +0.6 -0.6
solid stress Fraxetin 3 mM; solid stress N.D. +0.2 N.D. +0.9 -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -1.0 N.D. +0.7 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. -0.4 N.D. -0.1 +1.6
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.6 N.D. +0.2 +1.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.4 N.D. +0.9 +1.0
agar plate interaction control Taped volatile agar plate with no fungus N.D. -0.3 N.D. +0.4 +1.6
phage P. simiae OR1 MOI 0.1 N.D. +0.7 N.D. +0.7 +0.9
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.2 N.D. +0.2 +2.0
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