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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_27270 and PS417_27275 are separated by 3 nucleotides
PS417_27275 and PS417_27280 overlap by 8 nucleotides
PS417_27280 and PS417_27285 overlap by 4 nucleotides
PS417_27285 and PS417_27290 are separated by 19 nucleotides
PS417_27270: PS417_27270 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 5,886,714 to 5,887,937
_27270
PS417_27275: PS417_27275 - hypothetical protein, at 5,887,941 to 5,888,426
_27275
PS417_27280: PS417_27280 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 5,888,419 to 5,889,606
_27280
PS417_27285: PS417_27285 - Xaa-Pro aminopeptidase, at 5,889,603 to 5,890,925
_27285
PS417_27290: PS417_27290 - hypothetical protein, at 5,890,945 to 5,891,502
_27290
Group
Condition
PS417
_27270
PS417
_27275
PS417
_27280
PS417
_27285
PS417
_27290
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.3
N.D.
+0.8
-5.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.1
N.D.
-0.5
-5.2
solid stress
Fraxetin 2 mM; solid stress
N.D.
+0.1
N.D.
+0.5
-5.4
solid stress
Fraxetin 3 mM; solid stress
N.D.
+0.0
N.D.
+1.0
-5.2
supernatant control:fungal media
R2A with Vogels_fungal_media 0.2X
N.D.
-0.3
N.D.
+0.1
-3.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
-0.2
N.D.
-0.5
-2.8
carbon source
L-Valine (C)
N.D.
+0.2
N.D.
+0.2
-3.7
stress
Polymyxin B sulfate 0.006 mg/ml
N.D.
+0.1
N.D.
-3.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.7
N.D.
-0.5
-1.8
phage
JP1 MOI 10
N.D.
+0.2
N.D.
+0.0
-2.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
N.D.
-0.6
-1.5
phage
JP1 MOI 1
N.D.
+0.2
N.D.
+0.4
-3.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.4
N.D.
-0.1
-1.9
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.4
N.D.
-0.2
-1.6
phage
JP1 MOI 10
N.D.
-0.0
N.D.
-0.0
-2.1
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
N.D.
+0.4
N.D.
-0.5
-2.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-1.1
N.D.
+0.3
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.4
N.D.
-0.7
+0.2
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
+0.3
N.D.
-0.5
-1.5
phage
P. simiae OR Antrim MOI 1
N.D.
+0.3
N.D.
+0.1
-2.1
seeds
Growth on radish seeds for 72 hours
N.D.
+0.4
N.D.
-0.3
-1.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-1.1
N.D.
+0.6
-0.6
solid stress
Fraxetin 3 mM; solid stress
N.D.
+0.2
N.D.
+0.9
-1.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.0
N.D.
+0.7
+0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
-0.4
N.D.
-0.1
+1.6
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.6
N.D.
+0.2
+1.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-0.4
N.D.
+0.9
+1.0
agar plate interaction control
Taped volatile agar plate with no fungus
N.D.
-0.3
N.D.
+0.4
+1.6
phage
P. simiae OR1 MOI 0.1
N.D.
+0.7
N.D.
+0.7
+0.9
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
+0.2
N.D.
+0.2
+2.0
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