Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27260 and PS417_27265 are separated by 163 nucleotidesPS417_27265 and PS417_27270 are separated by 161 nucleotidesPS417_27270 and PS417_27275 are separated by 3 nucleotidesPS417_27275 and PS417_27280 overlap by 8 nucleotides PS417_27260: PS417_27260 - iron ABC transporter permease, at 5,883,768 to 5,885,384 _27260 PS417_27265: PS417_27265 - iron ABC transporter substrate-binding protein, at 5,885,548 to 5,886,552 _27265 PS417_27270: PS417_27270 - 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 5,886,714 to 5,887,937 _27270 PS417_27275: PS417_27275 - hypothetical protein, at 5,887,941 to 5,888,426 _27275 PS417_27280: PS417_27280 - 2-octaprenyl-6-methoxyphenyl hydroxylase, at 5,888,419 to 5,889,606 _27280
Group Condition PS417_27260 PS417_27265 PS417_27270 PS417_27275 PS417_27280
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -2.4 -2.3 N.D. +0.3 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -1.4 -1.1 N.D. +0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.6 N.D. -1.3 N.D.
stress L-Lysine 60 mM -0.8 -0.9 N.D. +0.1 N.D.
motility inner cut, LB soft agar motility assay -0.5 -0.5 N.D. -0.5 N.D.
motility outer cut, LB soft agar motility assay -0.2 -0.5 N.D. -0.7 N.D.
solid stress Fraxetin 3 mM; solid stress -0.8 -0.6 N.D. +0.0 N.D.
motility outer cut, LB soft agar motility assay -0.3 -0.3 N.D. -0.7 N.D.
motility inner cut, LB soft agar motility assay -0.3 -0.8 N.D. -0.2 N.D.
solid stress Fraxetin 2 mM; solid stress -0.4 -0.7 N.D. -0.2 N.D.
stress Choline Lysine 25 mM -0.6 -0.6 N.D. +0.1 N.D.
solid stress Fraxetin 3 mM; solid stress -0.7 -0.4 N.D. +0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.1 N.D. -0.8 N.D.
phage JP1 MOI 0.1 -0.6 -0.4 N.D. +0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.3 N.D. -1.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.3 +0.3 N.D. -0.6 N.D.
soil soil sample 7; outgrowth in LB -0.3 +0.3 N.D. -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.0 N.D. -0.8 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.0 N.D. -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.4 +0.2 N.D. -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +0.0 N.D. -1.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.3 +0.1 N.D. -0.7 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.5 -0.3 N.D. -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.5 +0.1 N.D. -0.6 N.D.
phage P. simiae OR1 MOI 0.1 -0.3 -0.1 N.D. +0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.6 N.D. -0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.0 +0.1 N.D. -0.2 N.D.
soil soil sample 8; outgrowth in LB +0.6 +0.5 N.D. -0.1 N.D.
rhizosphere rhizosphere sample 8; outgrowth in LB -0.2 +0.9 N.D. +0.4 N.D.
root root sample 6; outgrowth in LB +0.6 +0.5 N.D. +0.2 N.D.
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