Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27130 and PS417_27135 are separated by 58 nucleotidesPS417_27135 and PS417_27140 are separated by 597 nucleotidesPS417_27140 and PS417_27145 are separated by 220 nucleotidesPS417_27145 and PS417_27150 are separated by 109 nucleotides PS417_27130: PS417_27130 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (from data), at 5,856,571 to 5,857,935 _27130 PS417_27135: PS417_27135 - glutamine synthetase, at 5,857,994 to 5,859,352 _27135 PS417_27140: PS417_27140 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data), at 5,859,950 to 5,861,326 _27140 PS417_27145: PS417_27145 - TetR family transcriptional regulator, at 5,861,547 to 5,862,188 _27145 PS417_27150: PS417_27150 - transporter, at 5,862,298 to 5,862,513 _27150
Group Condition PS417_27130 PS417_27135 PS417_27140 PS417_27145 PS417_27150
carbon source Putrescine (C) -3.8 -4.7 -6.1 -0.5 N.D.
carbon source Putrescine (C) -4.0 -3.3 -4.9 -0.3 N.D.
nitrogen source Putrescine (N) -2.7 -2.2 -4.8 +0.1 N.D.
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.3 -3.6 -1.8 -0.1 N.D.
carbon source Carbon source L-Citrulline 5 mM -0.1 -4.1 -0.7 -0.3 N.D.
soil soil sample 5; outgrowth in LB -0.1 -3.5 -1.3 -0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -4.4 -0.3 +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -4.5 -0.2 -0.2 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -4.1 -0.4 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -4.5 -0.5 +0.2 N.D.
liquid stress Fraxetin 3 mM; liquid stress -0.1 -3.9 -0.7 +0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.0 -4.5 +0.1 -0.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -4.6 +0.2 -0.1 N.D.
liquid stress Scopoletin 3 mM; liquid stress -0.2 -3.8 -0.2 +0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -4.5 +0.5 +0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -2.0 -2.0 +0.3 N.D.
liquid stress Fraxetin 2 mM; liquid stress -0.1 -3.7 -0.2 +0.2 N.D.
solid stress Fraxetin 3 mM; solid stress -0.1 -4.1 +0.5 -0.1 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -4.1 +0.5 +0.0 N.D.
liquid stress Scopoletin 3 mM; liquid stress -0.1 -3.2 -0.3 +0.0 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -2.6 -0.3 -0.3 N.D.
liquid stress 80% Methanol 3 mM; liquid stress -0.1 -3.4 -0.0 +0.1 N.D.
phage P. simiae ORA MOI 10 +0.0 -3.7 +0.3 -0.0 N.D.
agar plate interaction control Taped volatile agar plate with no fungus +0.1 -4.2 +1.0 -0.2 N.D.
soil soil sample 8; outgrowth in LB -0.2 -1.7 -1.5 +0.0 N.D.
carbon source L-Isoleucine (C) -0.1 -3.3 +0.4 -0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -3.3 -0.2 +0.4 N.D.
carbon source L-Arginine (C) +0.1 +1.3 -1.7 +0.3 N.D.
stress Gentamicin 0.008 mg/ml +0.5 +3.0 -0.4 +0.1 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +2.0 +0.9 +0.1 +0.4 N.D.
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