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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_27125 and PS417_27130 are separated by 198 nucleotides
PS417_27130 and PS417_27135 are separated by 58 nucleotides
PS417_27135 and PS417_27140 are separated by 597 nucleotides
PS417_27140 and PS417_27145 are separated by 220 nucleotides
PS417_27125: PS417_27125 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,855,278 to 5,856,372
_27125
PS417_27130: PS417_27130 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19)
(from data)
, at 5,856,571 to 5,857,935
_27130
PS417_27135: PS417_27135 - glutamine synthetase, at 5,857,994 to 5,859,352
_27135
PS417_27140: PS417_27140 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11)
(from data)
, at 5,859,950 to 5,861,326
_27140
PS417_27145: PS417_27145 - TetR family transcriptional regulator, at 5,861,547 to 5,862,188
_27145
Group
Condition
PS417
_27125
PS417
_27130
PS417
_27135
PS417
_27140
PS417
_27145
carbon source
Putrescine (C)
-4.1
-3.8
-4.7
-6.1
-0.5
carbon source
Putrescine (C)
-4.1
-4.0
-3.3
-4.9
-0.3
nitrogen source
Putrescine (N)
-3.5
-2.7
-2.2
-4.8
+0.1
soil
soil sample 5; outgrowth in LB
-0.8
-0.1
-3.5
-1.3
-0.2
liquid stress
Fraxetin 3 mM; liquid stress
-0.9
-0.1
-3.9
-0.7
+0.1
carbon source
Carbon source L-Citrulline 5 mM
-0.1
-0.1
-4.1
-0.7
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
-0.3
-4.4
-0.3
+0.1
solid stress
Fraxetin 3 mM; solid stress
-1.2
-0.1
-4.1
+0.5
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.1
+0.1
-4.1
-0.4
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.4
-0.0
-4.5
+0.1
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.1
-4.5
-0.2
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.2
-2.0
-2.0
+0.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.9
-0.2
-4.1
+0.5
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.2
-4.5
-0.5
+0.2
liquid stress
Scopoletin 3 mM; liquid stress
-0.2
-0.2
-3.8
-0.2
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.2
-0.8
-2.4
-0.0
+0.0
liquid stress
Fraxetin 2 mM; liquid stress
-0.4
-0.1
-3.7
-0.2
+0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
+1.1
+0.3
-3.6
-1.8
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
+0.0
-4.6
+0.2
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
-0.0
-4.5
+0.5
+0.0
liquid stress
Scopoletin 3 mM; liquid stress
-0.3
-0.1
-3.2
-0.3
+0.0
liquid stress
80% Methanol 3 mM; liquid stress
-0.4
-0.1
-3.4
-0.0
+0.1
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
+0.1
-4.2
+1.0
-0.2
seeds
Growth on radish seeds for 72 hours
-1.0
-0.2
-2.7
+0.0
+0.2
phage
P. simiae ORA MOI 10
+0.0
+0.0
-3.7
+0.3
-0.0
carbon source
L-Isoleucine (C)
-0.2
-0.1
-3.3
+0.4
-0.1
rhizosphere
rhizosphere sample 2; outgrowth in LB
-1.0
+0.4
-2.6
+0.3
-0.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
+0.2
-3.3
-0.2
+0.4
solid stress
Fraxetin 3 mM; solid stress
-1.7
-0.2
+0.9
+1.2
+0.0
stress
Gentamicin 0.008 mg/ml
+1.0
+0.5
+3.0
-0.4
+0.1
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