Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27125 and PS417_27130 are separated by 198 nucleotidesPS417_27130 and PS417_27135 are separated by 58 nucleotidesPS417_27135 and PS417_27140 are separated by 597 nucleotidesPS417_27140 and PS417_27145 are separated by 220 nucleotides PS417_27125: PS417_27125 - spermidine/putrescine ABC transporter substrate-binding protein, at 5,855,278 to 5,856,372 _27125 PS417_27130: PS417_27130 - Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (from data), at 5,856,571 to 5,857,935 _27130 PS417_27135: PS417_27135 - glutamine synthetase, at 5,857,994 to 5,859,352 _27135 PS417_27140: PS417_27140 - Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data), at 5,859,950 to 5,861,326 _27140 PS417_27145: PS417_27145 - TetR family transcriptional regulator, at 5,861,547 to 5,862,188 _27145
Group Condition PS417_27125 PS417_27130 PS417_27135 PS417_27140 PS417_27145
carbon source Putrescine (C) -4.1 -3.8 -4.7 -6.1 -0.5
carbon source Putrescine (C) -4.1 -4.0 -3.3 -4.9 -0.3
nitrogen source Putrescine (N) -3.5 -2.7 -2.2 -4.8 +0.1
soil soil sample 5; outgrowth in LB -0.8 -0.1 -3.5 -1.3 -0.2
liquid stress Fraxetin 3 mM; liquid stress -0.9 -0.1 -3.9 -0.7 +0.1
carbon source Carbon source L-Citrulline 5 mM -0.1 -0.1 -4.1 -0.7 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.3 -4.4 -0.3 +0.1
solid stress Fraxetin 3 mM; solid stress -1.2 -0.1 -4.1 +0.5 -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.1 +0.1 -4.1 -0.4 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.4 -0.0 -4.5 +0.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.1 -4.5 -0.2 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.2 -2.0 -2.0 +0.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.9 -0.2 -4.1 +0.5 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.2 -4.5 -0.5 +0.2
liquid stress Scopoletin 3 mM; liquid stress -0.2 -0.2 -3.8 -0.2 +0.0
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.2 -0.8 -2.4 -0.0 +0.0
liquid stress Fraxetin 2 mM; liquid stress -0.4 -0.1 -3.7 -0.2 +0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +1.1 +0.3 -3.6 -1.8 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +0.0 -4.6 +0.2 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.0 -4.5 +0.5 +0.0
liquid stress Scopoletin 3 mM; liquid stress -0.3 -0.1 -3.2 -0.3 +0.0
liquid stress 80% Methanol 3 mM; liquid stress -0.4 -0.1 -3.4 -0.0 +0.1
agar plate interaction control Taped volatile agar plate with no fungus -0.4 +0.1 -4.2 +1.0 -0.2
seeds Growth on radish seeds for 72 hours -1.0 -0.2 -2.7 +0.0 +0.2
phage P. simiae ORA MOI 10 +0.0 +0.0 -3.7 +0.3 -0.0
carbon source L-Isoleucine (C) -0.2 -0.1 -3.3 +0.4 -0.1
rhizosphere rhizosphere sample 2; outgrowth in LB -1.0 +0.4 -2.6 +0.3 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.2 -3.3 -0.2 +0.4
solid stress Fraxetin 3 mM; solid stress -1.7 -0.2 +0.9 +1.2 +0.0
stress Gentamicin 0.008 mg/ml +1.0 +0.5 +3.0 -0.4 +0.1
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