Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_27075 and PS417_27080 are separated by 30 nucleotidesPS417_27080 and PS417_27085 overlap by 15 nucleotidesPS417_27085 and PS417_27090 are separated by 100 nucleotidesPS417_27090 and PS417_27095 overlap by 23 nucleotides PS417_27075: PS417_27075 - thiol-disulfide isomerase, at 5,844,750 to 5,845,325 _27075 PS417_27080: PS417_27080 - penicillin amidase, at 5,845,356 to 5,847,710 _27080 PS417_27085: PS417_27085 - AraC family transcriptional regulator, at 5,847,696 to 5,848,604 _27085 PS417_27090: PS417_27090 - hypothetical protein, at 5,848,705 to 5,849,058 _27090 PS417_27095: PS417_27095 - AraC family transcriptional regulator, at 5,849,036 to 5,850,001 _27095
Group Condition PS417_27075 PS417_27080 PS417_27085 PS417_27090 PS417_27095
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -3.0 +0.1 -0.2 -0.1 -0.3
soil soil sample 8; outgrowth in LB +0.4 -0.3 -2.8 -0.3 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 +0.3 -1.7 -0.1 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -2.2 +0.0 -0.1 -0.2 -0.4
seeds Growth on radish seeds for 72 hours -0.4 +0.1 -1.3 -0.2 -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.7 +0.0 -0.4 -0.1 -0.0
soil soil sample 3; outgrowth in LB -0.6 -0.0 -1.6 -0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 +0.3 -1.2 +0.1 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.3 -0.0 -0.6 +0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.9 +0.1 -0.1 +0.3 -0.2
liquid stress 80% Methanol 3 mM; liquid stress -1.7 +0.1 -0.2 -0.1 +0.1
stress TSB 0.5x with 6-methoxy-2(3H)-benzoxazolone 1250 uM; timepoint 1 -1.7 -0.1 +0.1 +0.1 -0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.6 +0.2 -1.2 -0.0 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -2.2 +0.1 +0.3 -0.0 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.9 +0.1 -0.8 +0.0 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.2 +0.4 -0.7 +0.1 -0.1
phage P. simiae OR Antrim MOI 0.1 +0.7 -0.0 -1.0 -0.3 -0.5
soil soil sample 7; outgrowth in LB +0.2 -0.4 -1.2 +0.0 +0.2
ecofab LB in EcoFAB_3.5mL -1.1 +0.1 +0.6 -0.1 -0.4
soil soil sample 6; outgrowth in LB +0.7 -0.6 -0.9 +0.2 -0.0
agar plate interaction Taped volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.1 -0.9 +0.3 -0.3
seeds Growth on radish seeds for 72 hours -0.7 +0.2 +1.1 +0.0 +0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 -0.1 -0.8 +0.1 +0.2
stress R2A with Fusaric 120 ug/mL +1.3 -0.4 -0.4 +0.2 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.2 +0.3 -0.2 -0.2 +0.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +2.3 -0.0 -0.4 -0.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.6 -0.1 -0.2 -0.3 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.2 -0.0 +0.8 +0.1 -0.0
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +1.6 -0.1 +0.5 -0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 +0.1 +1.2 -0.1 -0.2
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