Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26815 and PS417_26820 overlap by 8 nucleotidesPS417_26820 and PS417_26825 overlap by 1 nucleotidesPS417_26825 and PS417_26830 are separated by 71 nucleotidesPS417_26830 and PS417_26835 are separated by 29 nucleotides PS417_26815: PS417_26815 - peptidase M16, at 5,790,089 to 5,791,444 _26815 PS417_26820: PS417_26820 - peptidase M16, at 5,791,437 to 5,792,927 _26820 PS417_26825: PS417_26825 - 16S rRNA methyltransferase, at 5,792,927 to 5,793,535 _26825 PS417_26830: PS417_26830 - alpha/beta hydrolase, at 5,793,607 to 5,794,602 _26830 PS417_26835: PS417_26835 - 3-mercaptopyruvate sulfurtransferase, at 5,794,632 to 5,795,495 _26835
Group Condition PS417_26815 PS417_26820 PS417_26825 PS417_26830 PS417_26835
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -3.3 +0.1 N.D. +0.1 -1.0
soil soil sample 6; outgrowth in LB -1.9 -0.2 N.D. +0.2 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.4 -0.5 N.D. -0.1 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -2.2 -0.3 N.D. -0.1 -0.2
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x -2.3 -0.7 N.D. +0.2 +0.1
stress methylglyoxal 0.032 vol% -2.3 -0.1 N.D. -0.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.6 -0.3 N.D. +0.2 -0.8
phage P. simiae ORA MOI 0.1 -1.6 -0.1 N.D. +0.0 -0.4
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.7 -0.9 N.D. -0.1 -0.3
carbon source Trisodium citrate (C) -1.5 -0.9 N.D. +0.2 +0.2
solid stress Fraxetin 3 mM; solid stress -1.4 -0.6 N.D. +0.2 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.7 -0.2 N.D. +0.2 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +0.3 N.D. +0.1 -1.0
carbon source Trisodium citrate (C) -0.9 -1.0 N.D. +0.1 +0.1
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 +0.3 N.D. +0.0 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.6 +0.3 N.D. +0.0 -0.3
motility inner cut, LB soft agar motility assay -1.5 -0.6 N.D. +0.2 +0.6
phage P. simiae OR Antrim MOI 1 -1.2 -0.5 N.D. +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.5 N.D. -0.3 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.5 -0.2 N.D. +0.1 +0.7
motility outer cut, LB soft agar motility assay -1.1 -0.4 N.D. +0.1 +0.6
phage P. simiae ORA MOI 10 -1.5 +0.3 N.D. +0.1 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.4 +0.2 N.D. +0.1 +0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 +0.2 N.D. +0.3 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +0.4 N.D. +0.2 +0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.8 -0.2 N.D. +0.0 +1.8
seeds Growth on radish seeds for 72 hours +1.6 -0.7 N.D. +0.0 +0.2
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 -0.1 N.D. -0.2 +1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.3 +0.5 N.D. +0.6 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +4.5 +0.3 N.D. -0.4 -0.6
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