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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26810 and PS417_26815 are separated by 232 nucleotides
PS417_26815 and PS417_26820 overlap by 8 nucleotides
PS417_26820 and PS417_26825 overlap by 1 nucleotides
PS417_26825 and PS417_26830 are separated by 71 nucleotides
PS417_26810: PS417_26810 - cell division protein FtsY, at 5,788,471 to 5,789,856
_26810
PS417_26815: PS417_26815 - peptidase M16, at 5,790,089 to 5,791,444
_26815
PS417_26820: PS417_26820 - peptidase M16, at 5,791,437 to 5,792,927
_26820
PS417_26825: PS417_26825 - 16S rRNA methyltransferase, at 5,792,927 to 5,793,535
_26825
PS417_26830: PS417_26830 - alpha/beta hydrolase, at 5,793,607 to 5,794,602
_26830
Group
Condition
PS417
_26810
PS417
_26815
PS417
_26820
PS417
_26825
PS417
_26830
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-3.3
+0.1
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-2.4
-0.5
N.D.
-0.1
stress
methylglyoxal 0.032 vol%
N.D.
-2.3
-0.1
N.D.
-0.5
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
-2.3
-0.7
N.D.
+0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
-2.2
-0.3
N.D.
-0.1
carbon source
Trisodium citrate (C)
N.D.
-1.5
-0.9
N.D.
+0.2
soil
soil sample 6; outgrowth in LB
N.D.
-1.9
-0.2
N.D.
+0.2
motility
outer cut, LB soft agar motility assay
N.D.
-1.4
-0.4
N.D.
-0.1
motility
inner cut, LB soft agar motility assay
N.D.
-1.5
-0.6
N.D.
+0.2
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.4
-0.6
N.D.
+0.2
carbon source
Trisodium citrate (C)
N.D.
-0.9
-1.0
N.D.
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-1.6
-0.3
N.D.
+0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
-0.7
-0.9
N.D.
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.4
-0.2
N.D.
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
-1.7
-0.2
N.D.
+0.2
phage
P. simiae ORA MOI 0.1
N.D.
-1.6
-0.1
N.D.
+0.0
phage
Wcs_1
N.D.
-1.4
-0.1
N.D.
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-1.5
-0.2
N.D.
+0.1
phage
P. simiae OR Antrim MOI 1
N.D.
-1.2
-0.5
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.6
+0.3
N.D.
+0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-1.4
-0.0
N.D.
+0.3
phage
P. simiae ORA MOI 10
N.D.
-1.5
+0.3
N.D.
+0.1
liquid stress
Fraxetin 2 mM; liquid stress
N.D.
-1.4
+0.1
N.D.
+0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-1.3
+0.2
N.D.
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-1.1
+0.4
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
+1.0
-0.1
N.D.
-0.6
seeds
Growth on radish seeds for 72 hours
N.D.
+1.6
-0.7
N.D.
+0.0
carbon source
L-Valine (C)
N.D.
+1.2
+0.5
N.D.
-0.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+1.3
+0.5
N.D.
+0.6
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
+4.5
+0.3
N.D.
-0.4
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