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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26795 and PS417_26800 are separated by 129 nucleotides
PS417_26800 and PS417_26805 overlap by 4 nucleotides
PS417_26805 and PS417_26810 overlap by 4 nucleotides
PS417_26810 and PS417_26815 are separated by 232 nucleotides
PS417_26795: PS417_26795 - RNA polymerase sigma 70, at 5,785,797 to 5,786,651
_26795
PS417_26800: PS417_26800 - cell division protein FtsX, at 5,786,781 to 5,787,806
_26800
PS417_26805: PS417_26805 - cell division protein FtsE, at 5,787,803 to 5,788,474
_26805
PS417_26810: PS417_26810 - cell division protein FtsY, at 5,788,471 to 5,789,856
_26810
PS417_26815: PS417_26815 - peptidase M16, at 5,790,089 to 5,791,444
_26815
Group
Condition
PS417
_26795
PS417
_26800
PS417
_26805
PS417
_26810
PS417
_26815
phage
P. simiae OR1 MOI 0.1
N.D.
-1.0
-4.7
N.D.
+0.6
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.2
-2.0
N.D.
-1.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.5
-3.4
N.D.
-0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
-0.3
-3.4
N.D.
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.3
-0.5
N.D.
-3.3
soil
soil sample 7; outgrowth in LB
N.D.
-0.5
-2.8
N.D.
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.3
-2.2
N.D.
-1.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
-0.7
-1.4
N.D.
-1.3
rhizosphere
rhizosphere sample 8; outgrowth in LB
N.D.
-0.5
-1.1
N.D.
-1.3
root
root sample 6; outgrowth in LB
N.D.
-0.1
-3.4
N.D.
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.2
+0.4
N.D.
-2.4
soil
soil sample 3; outgrowth in LB
N.D.
-0.3
-2.3
N.D.
+0.8
nophagecontrol
Only library
N.D.
-0.4
-1.7
N.D.
+0.6
liquid stress
80% Methanol 3 mM; liquid stress
N.D.
-0.8
-1.4
N.D.
+0.8
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
N.D.
+0.2
+1.0
N.D.
-2.3
carbon source
L-Valine (C)
N.D.
-0.5
-1.6
N.D.
+1.2
phage
P. simiae OR Antrim MOI 10
N.D.
-0.7
-1.1
N.D.
+1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
N.D.
+0.1
+1.1
N.D.
-1.7
in planta
Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.1
-1.3
N.D.
+1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.3
+1.7
N.D.
-1.4
exudates
RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x
N.D.
-0.4
+1.4
N.D.
-1.0
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+1.9
+0.5
N.D.
-0.5
stress
Neomycin 0.04 mg/ml
N.D.
+1.3
+1.2
N.D.
+0.2
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.3
+1.0
N.D.
+1.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
N.D.
+1.0
+0.8
N.D.
+1.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+2.3
+1.3
N.D.
+0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+3.0
+2.6
N.D.
-1.0
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
-0.0
+1.4
N.D.
+4.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+3.3
+2.8
N.D.
-0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+3.5
+3.1
N.D.
+0.7
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