Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26795 and PS417_26800 are separated by 129 nucleotidesPS417_26800 and PS417_26805 overlap by 4 nucleotidesPS417_26805 and PS417_26810 overlap by 4 nucleotidesPS417_26810 and PS417_26815 are separated by 232 nucleotides PS417_26795: PS417_26795 - RNA polymerase sigma 70, at 5,785,797 to 5,786,651 _26795 PS417_26800: PS417_26800 - cell division protein FtsX, at 5,786,781 to 5,787,806 _26800 PS417_26805: PS417_26805 - cell division protein FtsE, at 5,787,803 to 5,788,474 _26805 PS417_26810: PS417_26810 - cell division protein FtsY, at 5,788,471 to 5,789,856 _26810 PS417_26815: PS417_26815 - peptidase M16, at 5,790,089 to 5,791,444 _26815
Group Condition PS417_26795 PS417_26800 PS417_26805 PS417_26810 PS417_26815
phage P. simiae OR1 MOI 0.1 N.D. -1.0 -4.7 N.D. +0.6
solid stress Fraxetin 3 mM; solid stress N.D. -1.2 -2.0 N.D. -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.5 -3.4 N.D. -0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 -3.4 N.D. -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.3 -0.5 N.D. -3.3
soil soil sample 7; outgrowth in LB N.D. -0.5 -2.8 N.D. -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 -2.2 N.D. -1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. -0.7 -1.4 N.D. -1.3
rhizosphere rhizosphere sample 8; outgrowth in LB N.D. -0.5 -1.1 N.D. -1.3
root root sample 6; outgrowth in LB N.D. -0.1 -3.4 N.D. +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.2 +0.4 N.D. -2.4
soil soil sample 3; outgrowth in LB N.D. -0.3 -2.3 N.D. +0.8
nophagecontrol Only library N.D. -0.4 -1.7 N.D. +0.6
liquid stress 80% Methanol 3 mM; liquid stress N.D. -0.8 -1.4 N.D. +0.8
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x N.D. +0.2 +1.0 N.D. -2.3
carbon source L-Valine (C) N.D. -0.5 -1.6 N.D. +1.2
phage P. simiae OR Antrim MOI 10 N.D. -0.7 -1.1 N.D. +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.1 +1.1 N.D. -1.7
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.1 -1.3 N.D. +1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 +1.7 N.D. -1.4
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x N.D. -0.4 +1.4 N.D. -1.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +1.9 +0.5 N.D. -0.5
stress Neomycin 0.04 mg/ml N.D. +1.3 +1.2 N.D. +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 +1.0 N.D. +1.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +1.0 +0.8 N.D. +1.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +2.3 +1.3 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +3.0 +2.6 N.D. -1.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.0 +1.4 N.D. +4.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +3.3 +2.8 N.D. -0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +3.5 +3.1 N.D. +0.7
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