Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26780 and PS417_26785 are separated by 71 nucleotidesPS417_26785 and PS417_26790 are separated by 74 nucleotidesPS417_26790 and PS417_26795 are separated by 108 nucleotidesPS417_26795 and PS417_26800 are separated by 129 nucleotides PS417_26780: PS417_26780 - thiamine biosynthesis protein ThiS, at 5,784,242 to 5,784,442 _26780 PS417_26785: PS417_26785 - membrane protein, at 5,784,514 to 5,784,891 _26785 PS417_26790: PS417_26790 - peptidoglycan transglycosylase, at 5,784,966 to 5,785,688 _26790 PS417_26795: PS417_26795 - RNA polymerase sigma 70, at 5,785,797 to 5,786,651 _26795 PS417_26800: PS417_26800 - cell division protein FtsX, at 5,786,781 to 5,787,806 _26800
Group Condition PS417_26780 PS417_26785 PS417_26790 PS417_26795 PS417_26800
solid stress Fraxetin 3 mM; solid stress N.D. -1.2 -0.3 N.D. -1.2
stress L-Lysine 60 mM N.D. -0.2 -0.2 N.D. -1.8
stress Gentamicin 0.008 mg/ml N.D. +0.2 -0.9 N.D. -1.3
phage P. simiae OR1 MOI 0.1 N.D. -0.3 -0.4 N.D. -1.0
phage P. simiae Grant Run MOI 0.1 N.D. -0.5 +0.0 N.D. -1.0
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X N.D. -0.1 -0.2 N.D. -1.2
phage P. simiae OR Antrim MOI 0.1 N.D. -0.7 +0.1 N.D. -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.0 -1.3 N.D. -0.2
liquid stress 80% Methanol 3 mM; liquid stress N.D. -0.3 +0.1 N.D. -1.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X N.D. +0.2 -0.3 N.D. -0.9
seeds Growth on radish seeds for 72 hours N.D. +0.3 -0.3 N.D. -0.8
phage P. simiae Grant Run MOI 10 N.D. +0.3 -0.5 N.D. -0.6
carbon source D-Glucosamine Hydrochloride (C) N.D. +0.3 -0.2 N.D. -0.9
phage JP1 MOI 10 N.D. +0.7 -0.3 N.D. -1.0
supernatant control Vogels_fungal_media 0.1X N.D. +0.5 +0.1 N.D. -0.9
phage P. simiae ORA MOI 0.1 N.D. -0.5 -0.3 N.D. +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.7 -0.4 N.D. -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. +0.4 +0.7 N.D. -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.0 +1.0 N.D. -0.5
phage P. simiae OR Antrim MOI 1 N.D. +1.0 +0.2 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.0 +0.2 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 +0.3 N.D. +0.8
phage JP1 MOI 1 N.D. +0.7 +0.9 N.D. -0.0
stress Neomycin 0.04 mg/ml N.D. +0.2 +0.3 N.D. +1.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.2 -0.2 N.D. +1.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. +1.5 +1.0 N.D. -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.1 -0.0 N.D. +3.0
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. +0.3 +0.3 N.D. +2.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. -0.0 +0.0 N.D. +3.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 +0.3 N.D. +3.5
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