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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26780 and PS417_26785 are separated by 71 nucleotides
PS417_26785 and PS417_26790 are separated by 74 nucleotides
PS417_26790 and PS417_26795 are separated by 108 nucleotides
PS417_26795 and PS417_26800 are separated by 129 nucleotides
PS417_26780: PS417_26780 - thiamine biosynthesis protein ThiS, at 5,784,242 to 5,784,442
_26780
PS417_26785: PS417_26785 - membrane protein, at 5,784,514 to 5,784,891
_26785
PS417_26790: PS417_26790 - peptidoglycan transglycosylase, at 5,784,966 to 5,785,688
_26790
PS417_26795: PS417_26795 - RNA polymerase sigma 70, at 5,785,797 to 5,786,651
_26795
PS417_26800: PS417_26800 - cell division protein FtsX, at 5,786,781 to 5,787,806
_26800
Group
Condition
PS417
_26780
PS417
_26785
PS417
_26790
PS417
_26795
PS417
_26800
solid stress
Fraxetin 3 mM; solid stress
N.D.
-1.2
-0.3
N.D.
-1.2
stress
L-Lysine 60 mM
N.D.
-0.2
-0.2
N.D.
-1.8
stress
Gentamicin 0.008 mg/ml
N.D.
+0.2
-0.9
N.D.
-1.3
phage
P. simiae OR1 MOI 0.1
N.D.
-0.3
-0.4
N.D.
-1.0
phage
P. simiae Grant Run MOI 0.1
N.D.
-0.5
+0.0
N.D.
-1.0
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
N.D.
-0.1
-0.2
N.D.
-1.2
phage
P. simiae OR Antrim MOI 0.1
N.D.
-0.7
+0.1
N.D.
-0.7
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.0
-1.3
N.D.
-0.2
liquid stress
80% Methanol 3 mM; liquid stress
N.D.
-0.3
+0.1
N.D.
-1.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
N.D.
+0.2
-0.3
N.D.
-0.9
seeds
Growth on radish seeds for 72 hours
N.D.
+0.3
-0.3
N.D.
-0.8
phage
P. simiae Grant Run MOI 10
N.D.
+0.3
-0.5
N.D.
-0.6
carbon source
D-Glucosamine Hydrochloride (C)
N.D.
+0.3
-0.2
N.D.
-0.9
phage
JP1 MOI 10
N.D.
+0.7
-0.3
N.D.
-1.0
supernatant control
Vogels_fungal_media 0.1X
N.D.
+0.5
+0.1
N.D.
-0.9
phage
P. simiae ORA MOI 0.1
N.D.
-0.5
-0.3
N.D.
+0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.7
-0.4
N.D.
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
+0.4
+0.7
N.D.
-0.8
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
-0.0
+1.0
N.D.
-0.5
phage
P. simiae OR Antrim MOI 1
N.D.
+1.0
+0.2
N.D.
-0.6
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.0
+0.2
N.D.
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
+0.4
+0.3
N.D.
+0.8
phage
JP1 MOI 1
N.D.
+0.7
+0.9
N.D.
-0.0
stress
Neomycin 0.04 mg/ml
N.D.
+0.2
+0.3
N.D.
+1.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+0.2
-0.2
N.D.
+1.9
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
+1.5
+1.0
N.D.
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.1
-0.0
N.D.
+3.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
+0.3
+0.3
N.D.
+2.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
-0.0
+0.0
N.D.
+3.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
+0.3
+0.3
N.D.
+3.5
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