Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26600 and PS417_26605 overlap by 4 nucleotidesPS417_26605 and PS417_26610 overlap by 8 nucleotidesPS417_26610 and PS417_26615 overlap by 1 nucleotidesPS417_26615 and PS417_26620 are separated by 0 nucleotides PS417_26600: PS417_26600 - malonate decarboxylase subunit gamma, at 5,750,413 to 5,751,183 _26600 PS417_26605: PS417_26605 - malonate decarboxylase subunit beta, at 5,751,180 to 5,752,022 _26605 PS417_26610: PS417_26610 - malonate decarboxylase subunit delta, at 5,752,015 to 5,752,314 _26610 PS417_26615: PS417_26615 - triphosphoribosyl-dephospho-CoA synthase, at 5,752,314 to 5,753,147 _26615 PS417_26620: PS417_26620 - malonate decarboxylase subunit alpha, at 5,753,148 to 5,754,818 _26620
Group Condition PS417_26600 PS417_26605 PS417_26610 PS417_26615 PS417_26620
phage JP1 MOI 10 -0.0 -3.3 N.D. -0.4 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -3.3 N.D. +0.1 +0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.4 -2.5 N.D. -0.5 +0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -3.5 N.D. +0.2 +0.2
phage P. simiae OR Antrim MOI 10 -0.4 -2.8 N.D. +0.1 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -3.5 N.D. +0.2 -0.0
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.1 -3.7 N.D. +0.5 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -3.2 N.D. +0.5 +0.1
liquid stress Fraxetin 3 mM; liquid stress -0.2 -2.7 N.D. -0.1 +0.0
phage JP1 MOI 1 -0.4 -2.4 N.D. -0.2 +0.2
nophagecontrol Only library +0.0 -2.5 N.D. -0.3 +0.0
carbon source Carbon source L-Ornithine 5 mM -0.0 -2.5 N.D. -0.2 +0.0
no phage control Time6-nophage -0.3 -3.1 N.D. +0.6 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 -1.4 N.D. -0.9 -0.0
soil soil sample 8; outgrowth in LB -0.1 -2.7 N.D. +0.1 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 -2.4 N.D. +0.2 +0.2
stress Phosphomycin 0.001 mg/ml +0.1 -2.0 N.D. -0.5 -0.0
solid stress Fraxetin 0.5 mM; solid stress +0.2 -2.1 N.D. -0.2 -0.1
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.8X; growth supplemented with 0.4X LB +0.2 -2.4 N.D. -0.0 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Aspartic Acid; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.5 -2.1 N.D. +0.2 +0.1
solid stress 80% Methanol 3 mM; solid stress +0.1 -2.4 N.D. +0.3 -0.1
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.0 -1.7 N.D. -0.6 +0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.1 -1.7 N.D. -0.7 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -1.8 N.D. +0.4 +0.1
solid stress 80% Methanol 1 mM; solid stress +0.2 -2.2 N.D. +0.3 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -2.1 N.D. +0.5 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 -2.3 N.D. +0.7 +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 +0.9 N.D. +1.2 -0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.4 +1.7 N.D. +0.7 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +2.2 N.D. +0.1 -0.0
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