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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26575 and PS417_26580 are separated by 106 nucleotides
PS417_26580 and PS417_26585 are separated by 1 nucleotides
PS417_26585 and PS417_26590 are separated by 67 nucleotides
PS417_26590 and PS417_26595 overlap by 4 nucleotides
PS417_26575: PS417_26575 - LysR family transcriptional regulator, at 5,746,671 to 5,747,591
_26575
PS417_26580: PS417_26580 - malonate transporter, at 5,747,698 to 5,748,462
_26580
PS417_26585: PS417_26585 - malonate carrier protein, at 5,748,464 to 5,748,868
_26585
PS417_26590: PS417_26590 - malonate decarboxylase subunit epsilon, at 5,748,936 to 5,749,835
_26590
PS417_26595: PS417_26595 - phosphoribosyl-dephospho-CoA transferase, at 5,749,832 to 5,750,419
_26595
Group
Condition
PS417
_26575
PS417
_26580
PS417
_26585
PS417
_26590
PS417
_26595
rhizosphere
rhizosphere sample 2; outgrowth in LB
-0.2
-1.0
-0.1
-0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.3
-0.6
-0.3
N.D.
stress
methylglyoxal 0.032 vol%
-0.7
+0.3
-0.6
-0.3
N.D.
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
-0.9
-0.2
-0.2
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
-0.9
+0.1
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.3
+0.4
-0.4
N.D.
stress
Gentamicin 0.008 mg/ml
+0.2
+0.2
-1.0
-0.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
+0.0
-0.6
-0.5
N.D.
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.4
-0.2
-0.3
-0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.6
+0.4
-0.4
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant
-0.1
+0.8
-0.6
-0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.8
-0.5
+0.4
N.D.
seeds
Growth on radish seeds for 72 hours
-0.1
-0.5
+1.1
-0.4
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.2
+1.0
-0.3
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.3
+0.6
-0.3
+0.3
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
+0.3
-0.5
+0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.3
+0.4
+0.3
-0.5
N.D.
carbon source
L-Valine (C)
+0.3
-0.4
+0.8
-0.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
+0.1
+1.1
-0.4
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.6
+0.5
+0.1
-0.6
N.D.
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.3
+0.3
+0.5
+0.4
N.D.
phage
P. simiae ORA MOI 0.1
-0.2
+0.3
+0.2
+0.6
N.D.
phage
P. simiae Grant Run MOI 10
-0.3
+0.4
+0.4
+0.3
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.4
+0.2
+0.7
+0.6
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+0.6
+0.8
-0.3
-0.0
N.D.
stress
L-Lysine 60 mM
-0.2
+0.3
+0.7
+0.3
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.3
-0.2
+0.2
+0.8
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.2
+0.6
+0.5
-0.0
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
+0.2
-0.3
+1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.5
+0.4
+0.1
+0.5
N.D.
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