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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26520 and PS417_26525 are separated by 221 nucleotides
PS417_26525 and PS417_26530 are separated by 226 nucleotides
PS417_26530 and PS417_26535 are separated by 32 nucleotides
PS417_26535 and PS417_26540 are separated by 272 nucleotides
PS417_26520: PS417_26520 - amino acid ABC transporter substrate-binding protein, at 5,735,619 to 5,736,410
_26520
PS417_26525: PS417_26525 - DEAD/DEAH box helicase, at 5,736,632 to 5,738,512
_26525
PS417_26530: PS417_26530 - hypothetical protein, at 5,738,739 to 5,739,317
_26530
PS417_26535: PS417_26535 - cytochrome B561, at 5,739,350 to 5,739,901
_26535
PS417_26540: PS417_26540 - adenosylmethionine-8-amino-7-oxononanoate aminotransferase, at 5,740,174 to 5,741,580
_26540
Group
Condition
PS417
_26520
PS417
_26525
PS417
_26530
PS417
_26535
PS417
_26540
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.0
-0.6
-1.5
-0.4
-2.4
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x
-0.2
-0.1
-2.7
-1.8
-0.1
carbon source
Carbon source D-Mannitol 5 mM
+0.1
-0.2
-3.8
+0.2
+0.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
-0.3
-3.0
-0.2
+0.2
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
+0.1
-0.1
-1.3
-2.0
-0.2
motility
outer cut, LB soft agar motility assay
-0.6
-0.4
-2.1
-0.3
+0.0
carbon source
L-Valine (C)
-0.1
-0.2
-1.8
-0.2
-1.0
stress
methylglyoxal 0.032 vol%
-0.7
-0.9
-1.5
-0.3
+0.3
phage
P. simiae ORA MOI 0.1
-0.8
+0.6
-2.2
-0.6
+0.1
phage
P. simiae OR1 MOI 0.1
-0.0
+0.4
-2.3
-0.7
-0.0
phage
P. simiae ORA MOI 1
-0.6
+0.4
-1.4
-0.9
-0.1
nitrogen source
Ammonium chloride (N)
+0.0
+0.1
+0.6
-0.4
-2.9
phage
JP1 MOI 0.1
-0.6
+0.5
-2.2
+0.4
-0.4
stress
Gentamicin 0.008 mg/ml
-0.0
-2.8
+0.8
-0.1
-0.1
phage
P. simiae OR Antrim MOI 0.1
-0.5
+1.2
-2.6
+0.2
-0.2
phage
P. simiae OR Antrim MOI 10
-0.9
+1.1
-1.0
-1.0
-0.1
no phage control
Time6-nophage
-0.2
+0.4
+0.4
-0.6
-1.7
stress
2-Furfuraldehyde 0.03 vol%
-0.2
+0.9
-2.5
+0.1
+0.1
nitrogen source
Adenine (N)
+0.2
+0.3
+0.3
-0.1
-2.3
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.5
+0.7
-1.7
-0.4
+0.4
phage
P. simiae ORA MOI 10
-0.7
+0.8
-1.4
-0.3
+0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.4
-0.3
+1.2
-0.4
-0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+0.6
-1.0
-1.3
+0.8
phage moi 100
Time6-phageFRS
-0.4
+0.4
+1.3
-0.0
-1.7
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.4
+1.0
-1.1
-0.4
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.6
+0.6
+0.8
-0.9
+0.4
phage
P. simiae OR Antrim MOI 1
-0.9
+0.8
+1.4
-0.2
-0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+1.9
-0.7
-0.5
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.0
+0.4
+2.8
-0.2
-0.8
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.2
+0.7
+2.2
-0.3
+0.1
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