Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26445 and PS417_26450 are separated by 19 nucleotidesPS417_26450 and PS417_26455 are separated by 210 nucleotidesPS417_26455 and PS417_26460 are separated by 95 nucleotidesPS417_26460 and PS417_26465 are separated by 39 nucleotides PS417_26445: PS417_26445 - ArsR family transcriptional regulator, at 5,719,591 to 5,720,586 _26445 PS417_26450: PS417_26450 - S-adenosylmethionine synthetase, at 5,720,606 to 5,721,796 _26450 PS417_26455: PS417_26455 - hypothetical protein, at 5,722,007 to 5,722,357 _26455 PS417_26460: PS417_26460 - NAD-dependent DNA ligase LigB, at 5,722,453 to 5,724,117 _26460 PS417_26465: PS417_26465 - hypothetical protein, at 5,724,157 to 5,724,552 _26465
Group Condition PS417_26445 PS417_26450 PS417_26455 PS417_26460 PS417_26465
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -1.3 N.D. -0.3 -0.1 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 N.D. -0.3 -0.4 -1.2
soil soil sample 4; outgrowth in LB +0.1 N.D. -0.5 +0.0 -1.6
rhizosphere rhizosphere sample 1; outgrowth in LB -1.0 N.D. -0.2 -0.3 -0.4
rhizosphere rhizosphere sample 8; outgrowth in LB -0.2 N.D. -0.3 -0.1 -1.2
phage P. simiae ORA MOI 1 -0.9 N.D. -0.6 -0.1 -0.2
soil soil sample 3; outgrowth in LB -0.5 N.D. +0.1 +0.1 -1.1
soil soil sample 5; outgrowth in LB -1.2 N.D. +0.1 -0.4 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 N.D. -1.2 -0.1 +0.3
seeds Growth on radish seeds for 24 hours +0.3 N.D. -0.3 +0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 N.D. -0.5 -0.4 +0.9
motility inner cut, LB soft agar motility assay +0.5 N.D. -0.4 -0.0 -0.8
soil soil sample 2; outgrowth in LB +0.0 N.D. -0.2 -1.0 +0.7
phage moi 100 Time6-phageFRS -0.2 N.D. -0.9 -0.1 +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 N.D. -0.5 -0.3 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +1.0 N.D. -0.0 -0.6 +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.6 N.D. -0.2 -0.2 -0.4
seeds Growth on radish seeds for 72 hours +1.3 N.D. -0.0 -0.3 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.5 N.D. -0.1 -0.4 +0.8
seeds Growth on radish seeds for 72 hours +1.1 N.D. +0.0 +0.3 -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.9 N.D. +2.5 +0.1 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.2 N.D. -0.5 +0.2 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 N.D. -0.6 +0.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.5 N.D. +1.7 +0.3 -0.0
stress Gentamicin 0.008 mg/ml -0.7 N.D. +0.1 -0.3 +2.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 N.D. +0.1 +0.5 +0.4
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.8 N.D. -0.1 +0.0 +1.3
nophagecontrol Only library +0.5 N.D. +1.1 +0.1 +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 N.D. +3.2 -0.0 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +3.9 N.D. -0.8 -0.5 +0.9
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