Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26320 and PS417_26325 overlap by 4 nucleotidesPS417_26325 and PS417_26330 are separated by 188 nucleotidesPS417_26330 and PS417_26335 are separated by 334 nucleotidesPS417_26335 and PS417_26340 are separated by 110 nucleotides PS417_26320: PS417_26320 - ABC transporter permease, at 5,688,238 to 5,689,083 _26320 PS417_26325: PS417_26325 - ABC transporter ATP-binding protein, at 5,689,080 to 5,690,258 _26325 PS417_26330: PS417_26330 - beta-aspartyl peptidase, at 5,690,447 to 5,692,438 _26330 PS417_26335: PS417_26335 - BetI family transcriptional regulator, at 5,692,773 to 5,693,366 _26335 PS417_26340: PS417_26340 - betaine-aldehyde dehydrogenase, at 5,693,477 to 5,694,949 _26340
Group Condition PS417_26320 PS417_26325 PS417_26330 PS417_26335 PS417_26340
stress Paraquat dichloride 0.002 mg/ml +0.2 +1.0 +2.0 -5.5 -3.2
phage P. simiae OR Antrim MOI 1 -0.2 -0.2 +0.1 -3.7 -0.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 -0.0 +0.0 -2.3 -1.3
phage P. simiae ORA MOI 0.1 -0.3 +0.1 -0.1 -0.5 -2.3
liquid stress 80% Methanol 2 mM; liquid stress -0.1 +0.1 +0.0 -2.5 -0.4
stress Choline chloride 300 mM +0.1 -0.3 -0.4 -0.8 -1.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.4 -0.4 -0.7 -0.7
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.2 -0.3 -0.6 -1.3 -0.6
seeds Growth on radish seeds for 72 hours -0.5 +0.2 -0.4 -1.1 -0.8
solid stress Fraxetin 3 mM; solid stress +0.1 -0.5 -0.2 -1.4 -0.5
phage JP1 MOI 10 -0.1 -0.1 +0.5 -1.7 -1.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.3 +0.4 +0.0 -1.3 -1.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 -0.4 +0.4 -1.1 -1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.3 +0.3 +0.2 -0.9 -1.6
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 +0.2 +0.4 -1.4 -1.0
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB +0.2 -0.3 -0.4 -1.6 +0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 +0.0 +0.8 -1.4 -1.1
phage P. simiae ORA MOI 0.1 +0.1 -0.1 +0.0 +0.8 -2.2
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.6 -0.2 -1.4 +0.8
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.3 +0.5 -1.4 -0.8
stress Paraquat dichloride 0.008 mg/ml +0.3 +2.4 +4.4 -4.6 -3.4
stress Thallium(I) acetate 0.0625 mg/ml +0.5 +2.1 +3.3 -3.5 -3.0
stress 1-ethyl-3-methylimidazolium acetate 35 mM -0.2 +0.1 -0.2 -1.5 +1.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.4 -0.3 +0.8 -1.1 -0.2
soil soil sample 5; outgrowth in LB +1.3 +0.9 +0.0 -0.1 -0.9
rhizosphere rhizosphere sample 1; outgrowth in LB +0.8 +1.3 +0.9 -0.3 -0.3
soil soil sample 6; outgrowth in LB +1.2 +1.0 +0.1 +1.0 -0.9
soil soil sample 7; outgrowth in LB +0.9 +0.6 +0.4 +0.5 +0.4
root root sample 6; outgrowth in LB +1.4 +1.2 +0.4 +0.5 -0.4
soil soil sample 4; outgrowth in LB +2.5 +2.3 +0.1 -1.0 -0.2
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