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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26280 and PS417_26285 are separated by 142 nucleotides
PS417_26285 and PS417_26290 are separated by 14 nucleotides
PS417_26290 and PS417_26295 are separated by 27 nucleotides
PS417_26295 and PS417_26300 are separated by 360 nucleotides
PS417_26280: PS417_26280 - NADPH:quinone reductase, at 5,679,418 to 5,680,305
_26280
PS417_26285: PS417_26285 - 3-hydroxybutyryl-CoA dehydrogenase, at 5,680,448 to 5,681,413
_26285
PS417_26290: PS417_26290 - 4-hydroxybenzoyl-CoA thioesterase, at 5,681,428 to 5,681,904
_26290
PS417_26295: PS417_26295 - gamma-butyrobetaine dioxygenase, at 5,681,932 to 5,683,092
_26295
PS417_26300: PS417_26300 - hypothetical protein, at 5,683,453 to 5,683,716
_26300
Group
Condition
PS417
_26280
PS417
_26285
PS417
_26290
PS417
_26295
PS417
_26300
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.9
-0.1
-0.2
-1.1
-0.6
soil
soil sample 2; outgrowth in LB
-0.1
-0.7
-0.8
-0.4
-0.2
soil
soil sample 4; outgrowth in LB
-0.2
-0.5
-0.4
-0.5
-0.5
root
root sample 6; outgrowth in LB
-0.3
+0.0
-0.0
-1.2
-0.5
rhizosphere
rhizosphere sample 2; outgrowth in LB
+0.1
-0.4
-0.3
+0.3
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.6
-0.4
-0.7
-1.2
+0.1
motility
outer cut, LB soft agar motility assay
-0.5
-0.4
-0.4
+0.4
-0.5
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
-1.0
+0.2
+0.3
-0.3
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.5
-0.4
-0.1
+0.4
-0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-0.5
+0.6
-0.4
-0.7
-0.1
soil
soil sample 7; outgrowth in LB
-1.2
-0.3
+0.4
+0.2
-0.1
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
+0.2
-0.4
+0.5
-0.3
soil
soil sample 8; outgrowth in LB
-0.8
+0.6
+0.1
+0.2
-0.4
phage
JP1 MOI 10
+0.5
-0.2
-0.0
-1.1
+0.5
carbon source
RCH2_defined_glucose with MS_media 1x
-0.7
+0.1
+0.1
+0.8
-0.4
stress
Doxycycline hyclate 0.001 mg/ml
+0.3
-0.6
+0.9
-0.3
-0.3
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.5
+0.2
+0.3
-0.8
-0.2
agar plate interaction control
Taped volatile agar plate with no fungus
-0.4
-0.2
+0.3
+0.8
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.1
+0.2
+0.6
-0.9
phage
P. simiae OR Antrim MOI 1
+0.7
-0.4
+0.7
-0.3
-0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
+0.3
-0.1
+0.7
+0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.5
+0.7
+0.0
+1.0
-0.4
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.5
+0.2
+0.8
+0.7
-0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.3
-0.2
+0.4
+1.1
-0.4
phage
P. simiae OR1 MOI 0.1
+0.5
+0.3
-0.7
+0.7
+0.6
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.2
-0.1
+1.9
+0.4
-0.5
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.3
+0.2
+0.9
+0.3
+0.6
solid stress
80% Methanol 1 mM; solid stress
+1.0
+0.4
-0.1
+0.3
+0.2
carbon source
L-Valine (C)
+1.8
-0.3
+0.0
+0.6
+0.2
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+1.7
+0.3
-0.1
+0.7
+0.0
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