Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26270 and PS417_26275 are separated by 207 nucleotidesPS417_26275 and PS417_26280 are separated by 73 nucleotidesPS417_26280 and PS417_26285 are separated by 142 nucleotidesPS417_26285 and PS417_26290 are separated by 14 nucleotides PS417_26270: PS417_26270 - AraC family transcriptional regulator, at 5,677,248 to 5,678,192 _26270 PS417_26275: PS417_26275 - glycine/betaine ABC transporter substrate-binding protein, at 5,678,400 to 5,679,344 _26275 PS417_26280: PS417_26280 - NADPH:quinone reductase, at 5,679,418 to 5,680,305 _26280 PS417_26285: PS417_26285 - 3-hydroxybutyryl-CoA dehydrogenase, at 5,680,448 to 5,681,413 _26285 PS417_26290: PS417_26290 - 4-hydroxybenzoyl-CoA thioesterase, at 5,681,428 to 5,681,904 _26290
Group Condition PS417_26270 PS417_26275 PS417_26280 PS417_26285 PS417_26290
phage Wcs_1 -1.3 -0.8 -0.5 -0.3 +0.1
agar plate interaction control Taped volatile agar plate with no fungus -0.7 -0.8 -0.4 -0.2 +0.3
motility inner cut, LB soft agar motility assay -1.0 +0.4 -0.6 -0.1 -0.4
supernatant control Vogels_fungal_media 0.1X -0.6 -0.4 -0.8 +0.5 -0.3
soil soil sample 4; outgrowth in LB +0.4 -0.8 -0.2 -0.5 -0.4
motility outer cut, LB soft agar motility assay -0.4 +0.5 -0.7 -0.6 -0.0
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.6 +0.6 -0.6 +0.2 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 -0.1 -0.5 -0.3 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.1 -0.4 -0.9 -0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +0.8 -0.3 +0.0 +0.2
soil soil sample 7; outgrowth in LB +0.8 +0.7 -1.2 -0.3 +0.4
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.5x +0.7 -0.3 -0.7 +0.1 +0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.5 -0.1 -0.7 +0.3 -0.1
seeds Growth on radish seeds for 72 hours -0.4 +1.3 -0.3 +0.3 +0.3
stress Doxycycline hyclate 0.001 mg/ml +0.2 +0.4 +0.3 -0.6 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.4 -0.3 +1.7 +0.3 -0.1
seeds Growth on radish seeds for 24 hours -0.6 +1.2 -0.0 +0.2 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.6 +0.1 -0.5 +0.2 +0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.5 -0.9 +0.4 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.5 +1.3 -0.3 +0.0 -0.2
phage Wcs_0.1 +1.0 +0.3 +0.1 -0.4 +0.4
carbon source L-Valine (C) +0.0 -0.1 +1.8 -0.3 +0.0
phage P. simiae OR Antrim MOI 1 +0.1 +0.3 +0.7 -0.4 +0.7
phage P. simiae OR1 MOI 0.1 +0.3 +1.3 +0.5 +0.3 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.7 +0.8 -0.3 +0.3 +0.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.9 +1.2 +0.0 -0.0 -0.2
solid stress 80% Methanol 1 mM; solid stress +0.6 +0.1 +1.0 +0.4 -0.1
solid stress Fraxetin 1 mM; solid stress +0.4 +0.7 +0.7 +0.4 -0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -0.2 +0.7 -0.2 -0.1 +1.9
phage P. simiae ORA MOI 0.1 +1.3 +0.1 +0.2 +0.4 +0.3
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