Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26170 and PS417_26175 are separated by 10 nucleotidesPS417_26175 and PS417_26180 are separated by 123 nucleotidesPS417_26180 and PS417_26185 overlap by 4 nucleotidesPS417_26185 and PS417_26190 are separated by 15 nucleotides PS417_26170: PS417_26170 - formyltetrahydrofolate deformylase, at 5,654,431 to 5,655,288 _26170 PS417_26175: PS417_26175 - sarcosine oxidase subunit gamma, at 5,655,299 to 5,655,931 _26175 PS417_26180: PS417_26180 - sarcosine oxidase subunit alpha, at 5,656,055 to 5,659,072 _26180 PS417_26185: PS417_26185 - sarcosine oxidase subunit delta, at 5,659,069 to 5,659,371 _26185 PS417_26190: PS417_26190 - sarcosine oxidase subunit beta, at 5,659,387 to 5,660,637 _26190
Group Condition PS417_26170 PS417_26175 PS417_26180 PS417_26185 PS417_26190
root root sample 6; outgrowth in LB +0.2 -1.7 -1.2 -1.7 -1.2
rhizosphere rhizosphere sample 1; outgrowth in LB -0.2 -1.9 -0.7 -0.8 -1.2
soil soil sample 6; outgrowth in LB +0.2 -2.8 -0.5 -0.5 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -3.3 -0.2 -0.2 -0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.4 -1.4 -0.2 -0.3 -0.8
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x +0.0 -1.1 -0.6 -0.4 -0.9
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.1 -1.0 -0.3 -0.6 -0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 -1.9 -0.1 +0.4 -0.9
solid stress Fraxetin 2 mM; solid stress +0.2 -2.4 +0.1 -0.2 -0.1
motility outer cut, LB soft agar motility assay -0.3 -1.8 +0.1 -0.2 -0.2
phage P. simiae ORA MOI 10 +0.2 -1.8 -0.3 +0.0 -0.6
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.4 -1.5 -0.5 -0.2 -0.5
phage P. simiae OR1 MOI 0.1 +0.3 -2.5 -0.3 +0.1 +0.3
carbon source L-Valine (C) +0.3 -1.9 +0.0 +0.2 -0.4
soil soil sample 5; outgrowth in LB +0.3 -1.4 -0.2 +0.5 -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -1.6 +0.3 +0.0 -0.2
phage P. simiae OR Antrim MOI 0.1 +0.5 -1.9 +0.3 +0.5 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +1.3 +0.2 -1.3 -0.0
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 -1.3 +0.2 +0.8 +0.5
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -1.4 +0.1 +0.1 +1.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 +1.7 -0.1 -0.5 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.0 +2.5 -0.3 -0.6 -0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.0 -0.9 -0.1 +1.2 +1.0
seeds Growth on radish seeds for 72 hours -0.0 +2.8 -0.1 -0.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 +2.1 +0.3 +0.1 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +1.8 -0.2 +0.5 -0.2
supernatant control Vogels_fungal_media 0.1X +0.2 +2.2 +0.2 -0.1 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +2.0 +0.0 +0.5 +0.2
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +2.4 +0.2 +0.3 +0.3
phage P. simiae ORA MOI 0.1 +0.2 +1.8 +0.2 +0.2 +0.7
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