Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26105 and PS417_26110 overlap by 4 nucleotidesPS417_26110 and PS417_26115 are separated by 107 nucleotidesPS417_26115 and PS417_26120 are separated by 79 nucleotidesPS417_26120 and PS417_26125 are separated by 34 nucleotides PS417_26105: PS417_26105 - ABC transporter permease, at 5,642,979 to 5,643,698 _26105 PS417_26110: PS417_26110 - ABC transporter permease, at 5,643,695 to 5,644,405 _26110 PS417_26115: PS417_26115 - glutamine ABC transporter ATP-binding protein, at 5,644,513 to 5,645,238 _26115 PS417_26120: PS417_26120 - hypothetical protein, at 5,645,318 to 5,645,935 _26120 PS417_26125: PS417_26125 - glutathione S-transferase, at 5,645,970 to 5,646,599 _26125
Group Condition PS417_26105 PS417_26110 PS417_26115 PS417_26120 PS417_26125
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.0 -0.1 -0.4 -0.9 -0.6
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.2 +0.2 -0.4 -1.4 -0.0
rhizosphere rhizosphere sample 1; outgrowth in LB -0.0 +0.4 -0.1 -1.8 -0.1
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.2 +0.8 -1.7 -0.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 +0.1 +0.5 -2.9 -0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 -0.1 +0.3 -1.0 -0.3
seeds Growth on radish seeds for 72 hours -0.3 -0.1 +0.7 -2.1 +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -0.6 +0.0 -0.6 +0.4 -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.1 +0.3 -1.2 +0.4
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.1 -0.1 +0.6 -1.3 +0.3
nophagecontrol Only library -0.2 +0.2 +0.1 -1.3 +0.5
phage JP1 MOI 1 +0.4 +0.3 -1.0 -0.6 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.3 -0.0 +0.6 -1.3 +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.2 +0.1 +0.1 -1.0 +0.7
carbon source L-Valine (C) -0.6 +0.1 -0.7 -0.6 +1.4
stress Nalidixic 0.015 mg/ml -0.4 +0.1 -0.5 +1.2 -0.2
supernatant control Vogels_fungal_media 0.1X +0.3 +0.0 -0.6 +0.7 +0.5
carbon source L-Valine (C) +0.3 +0.1 +0.1 -0.9 +1.5
soil soil sample 5; outgrowth in LB +0.3 +0.4 +0.7 -0.6 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.4 +0.1 +0.2 +0.1 +1.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 -0.1 +1.8 -0.2 -0.3
exudates RCH2_defined_glucose with Brachypodium distachyon 27-day exudates 1x +0.1 +0.0 +0.4 +1.2 -0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 -0.1 +0.6 -0.6 +1.8
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.3 +0.7 +0.0 +1.0
phage JP1 MOI 0.1 -0.0 +0.3 -0.3 +1.5 +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.1 +0.0 +0.6 +0.8 +0.6
phage P. simiae Grant Run MOI 0.1 +0.4 -0.0 +0.1 +1.0 +0.7
phage P. simiae ORA MOI 0.1 +0.5 -0.1 +0.5 +1.4 +0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.0 +0.5 +1.8 +0.5 +0.1
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.2 +0.2 +0.3 +3.6 -0.3
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