Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_26080 and PS417_26085 are separated by 63 nucleotidesPS417_26085 and PS417_26095 are separated by 302 nucleotidesPS417_26095 and PS417_26100 are separated by 8 nucleotidesPS417_26100 and PS417_26105 are separated by 65 nucleotides PS417_26080: PS417_26080 - TonB-dependent receptor, at 5,637,216 to 5,639,645 _26080 PS417_26085: PS417_26085 - peptidase, at 5,639,709 to 5,640,812 _26085 PS417_26095: PS417_26095 - ornithine cyclodeaminase, at 5,641,115 to 5,642,143 _26095 PS417_26100: PS417_26100 - amino acid ABC transporter, at 5,642,152 to 5,642,913 _26100 PS417_26105: PS417_26105 - ABC transporter permease, at 5,642,979 to 5,643,698 _26105
Group Condition PS417_26080 PS417_26085 PS417_26095 PS417_26100 PS417_26105
stress methylglyoxal 0.032 vol% -0.1 -1.0 -1.3 +0.1 +0.2
stress Gentamicin 0.008 mg/ml -0.1 -0.2 -0.8 -0.6 -0.2
nophagecontrol Only library -0.2 -1.3 +0.1 +0.1 -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.9 +0.2 -0.4 +0.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.5 +0.4 -0.7 -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamic; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.1 -0.4 +0.4 -0.4 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.5 +0.1 +0.4 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.6 +0.3 +0.0 -0.6
phage JP1 MOI 1 -0.0 -0.9 +0.1 -0.2 +0.4
ecofab LB 0.5x in EcoFAB_3.5mL +0.1 -1.0 +0.5 -0.0 -0.2
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days -0.2 +0.2 +0.5 -0.6 -0.3
phage P. simiae OR1 MOI 0.1 +0.0 -1.2 +0.8 +0.1 -0.1
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.1 -0.8 -0.2 +0.0 +0.6
phage P. simiae OR Antrim MOI 0.1 -0.1 -0.5 -0.5 +0.4 +0.3
motility inner cut, LB soft agar motility assay +0.0 +0.7 -0.3 -0.7 +0.3
soil soil sample 8; outgrowth in LB +0.4 -0.7 -0.3 +0.4 +0.4
soil soil sample 3; outgrowth in LB +0.1 -0.2 -0.4 +0.8 -0.2
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.0 +0.3 +0.4 -0.7 +0.2
rhizosphere rhizosphere sample 2; outgrowth in LB +0.2 +0.3 -0.8 +0.6 -0.2
carbon source L-Valine (C) -0.2 +0.5 +0.4 +0.1 -0.6
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.5 -0.3 -0.4 +0.4 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.0 -0.2 +0.7 +0.5 -0.4
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.2 +0.5 +0.3 +0.1 -0.4
phage Wcs_1 -0.1 +0.6 -0.6 +0.9 +0.1
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 -0.4 +0.1 -0.0 +1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 +1.3 -0.1 +0.0 -0.2
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.8 -0.4 +0.2 +0.3 +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.6 +0.1 +0.3 +0.7
soil soil sample 5; outgrowth in LB +0.1 +0.6 +0.3 +0.4 +0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.0 +0.5 +0.2 +0.2 +0.9
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