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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_26070 and PS417_26075 are separated by 109 nucleotides
PS417_26075 and PS417_26080 are separated by 146 nucleotides
PS417_26080 and PS417_26085 are separated by 63 nucleotides
PS417_26085 and PS417_26095 are separated by 302 nucleotides
PS417_26070: PS417_26070 - amino acid ABC transporter substrate-binding protein, at 5,633,151 to 5,634,146
_26070
PS417_26075: PS417_26075 - hypothetical protein, at 5,634,256 to 5,637,069
_26075
PS417_26080: PS417_26080 - TonB-dependent receptor, at 5,637,216 to 5,639,645
_26080
PS417_26085: PS417_26085 - peptidase, at 5,639,709 to 5,640,812
_26085
PS417_26095: PS417_26095 - ornithine cyclodeaminase, at 5,641,115 to 5,642,143
_26095
Group
Condition
PS417
_26070
PS417
_26075
PS417
_26080
PS417
_26085
PS417
_26095
stress
methylglyoxal 0.032 vol%
-0.4
-0.4
-0.1
-1.0
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.7
-0.1
-0.2
-0.9
+0.2
phage
P. simiae OR Antrim MOI 1
-0.6
-0.4
-0.1
-0.3
-0.3
nophagecontrol
Only library
+0.1
-0.1
-0.2
-1.3
+0.1
phage
JP1 MOI 1
-0.2
-0.4
-0.0
-0.9
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X
-0.8
-0.2
+0.1
-0.3
+0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.8
-0.2
+0.1
+0.2
-0.3
phage
P. simiae Grant Run MOI 10
-0.7
-0.4
-0.1
-0.0
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.4
-0.1
+0.1
-0.7
-0.5
agar plate interaction control
Taped volatile agar plate with no fungus
+0.5
-0.0
+0.0
-1.1
-0.0
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.2
+0.5
-0.3
-0.4
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.4
-0.0
-0.1
-0.6
+0.6
phage
JP1 MOI 10
-0.6
-0.3
-0.1
+0.4
+0.1
ecofab
LB 0.5x in EcoFAB_3.5mL
+0.0
-0.0
+0.1
-1.0
+0.5
motility
outer cut, LB soft agar motility assay
-0.5
+0.2
+0.1
+0.3
-0.5
phage
Wcs_1
+0.1
-0.4
-0.1
+0.6
-0.6
soil
soil sample 8; outgrowth in LB
+0.4
+0.1
+0.4
-0.7
-0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.5
-0.3
+0.8
-0.4
+0.2
phage
P. simiae OR Antrim MOI 10
-0.8
+0.0
-0.0
+0.6
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-0.2
+0.2
-0.6
+0.3
carbon source
L-Valine (C)
-0.6
-0.1
-0.2
+0.5
+0.4
phage
JP1 MOI 10
+0.5
+0.1
-0.0
-0.7
+0.2
phage
P. simiae OR1 MOI 0.1
+0.4
+0.1
+0.0
-1.2
+0.8
root
root sample 6; outgrowth in LB
-0.7
+0.0
+0.4
+0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.5
+0.0
-0.2
+0.4
+0.4
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
-0.7
+0.0
+0.5
+0.3
+0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
+0.3
+0.6
+0.2
+0.1
soil
soil sample 2; outgrowth in LB
-0.1
+0.1
+0.4
+0.7
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
+0.1
+0.1
+1.3
-0.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.4
+0.4
+0.2
+0.3
+0.2
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