Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02545 and PS417_02550 are separated by 152 nucleotidesPS417_02550 and PS417_02555 are separated by 225 nucleotidesPS417_02555 and PS417_02560 overlap by 4 nucleotidesPS417_02560 and PS417_02565 are separated by 309 nucleotides PS417_02545: PS417_02545 - tRNA-Leu, at 581,401 to 581,487 _02545 PS417_02550: PS417_02550 - tRNA-Leu, at 581,640 to 581,726 _02550 PS417_02555: PS417_02555 - exoribonuclease R, at 581,952 to 584,579 _02555 PS417_02560: PS417_02560 - 23S rRNA methyltransferase, at 584,576 to 585,331 _02560 PS417_02565: PS417_02565 - 30S ribosomal protein S6, at 585,641 to 586,063 _02565
Group Condition PS417_02545 PS417_02550 PS417_02555 PS417_02560 PS417_02565
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -1.6 -4.6 N.D.
phage JP1 MOI 1 N.D. N.D. -3.4 -2.8 N.D.
motility outer cut, LB soft agar motility assay N.D. N.D. -2.5 -3.3 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. -1.0 -4.5 N.D.
phage JP1 MOI 0.1 N.D. N.D. -3.0 -2.5 N.D.
nitrogen source Adenine (N) N.D. N.D. -4.6 -0.8 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -0.9 -4.5 N.D.
phage JP1 MOI 10 N.D. N.D. -2.8 -2.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.7 -2.6 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -0.8 -4.3 N.D.
solid stress 80% Methanol 2 mM; solid stress N.D. N.D. -1.9 -3.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.0 -2.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -2.9 -1.7 N.D.
stress Choline Lysine 25 mM N.D. N.D. -4.0 -0.5 N.D.
phage P. simiae OR Antrim MOI 1 N.D. N.D. -2.7 -1.6 N.D.
phage JP1 MOI 10 N.D. N.D. -2.5 -1.8 N.D.
stress methylglyoxal 0.032 vol% N.D. N.D. -4.9 +0.6 N.D.
motility_chemotaxis Method=Plug_approach; Chemical=None; Chemical_conc=0mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. N.D. -1.4 -2.9 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. -1.3 -2.8 N.D.
carbon source octanoate (C) N.D. N.D. -4.3 +0.1 N.D.
phage P. simiae ORA MOI 0.1 N.D. N.D. -2.0 -1.9 N.D.
soil soil sample 5; outgrowth in LB N.D. N.D. +0.4 -4.0 N.D.
stress L-Lysine 60 mM N.D. N.D. -3.8 +0.4 N.D.
carbon source octanoate (C) N.D. N.D. -4.2 +0.7 N.D.
soil soil sample 4; outgrowth in LB N.D. N.D. +1.3 -4.0 N.D.
phage P. simiae OR Antrim MOI 10 N.D. N.D. -3.0 +1.0 N.D.
stress Doxycycline hyclate 0.001 mg/ml N.D. N.D. -3.5 +1.8 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. N.D. -2.5 +1.5 N.D.
solid stress Fraxetin 3 mM; solid stress N.D. N.D. -2.4 +2.1 N.D.
stress Phosphomycin 0.003 mg/ml N.D. N.D. +3.4 -0.6 N.D.
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