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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_25655 and PS417_25660 are separated by 165 nucleotides
PS417_25660 and PS417_25665 are separated by 136 nucleotides
PS417_25665 and PS417_25670 are separated by 24 nucleotides
PS417_25670 and PS417_25675 are separated by 70 nucleotides
PS417_25655: PS417_25655 - iron--sulfur cluster insertion protein ErpA, at 5,542,382 to 5,542,732
_25655
PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932
_25660
PS417_25665: PS417_25665 - membrane protein, at 5,544,069 to 5,544,497
_25665
PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484
_25670
PS417_25675: PS417_25675 - membrane protein, at 5,545,555 to 5,546,511
_25675
Group
Condition
PS417
_25655
PS417
_25660
PS417
_25665
PS417
_25670
PS417
_25675
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-1.3
-0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-1.2
-0.6
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.8
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
N.D.
N.D.
N.D.
-0.5
-0.6
soil
soil sample 3; outgrowth in LB
N.D.
N.D.
N.D.
-0.9
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.4
-0.5
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
+0.0
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.5
-0.4
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
N.D.
N.D.
N.D.
-0.4
-0.5
no stress control on plate
growth on LB_noSalt plate
N.D.
N.D.
N.D.
-0.4
-0.5
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
N.D.
N.D.
N.D.
-0.5
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
+0.1
-0.8
phage
JP1 MOI 1
N.D.
N.D.
N.D.
-0.7
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
+0.2
-0.7
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
N.D.
N.D.
N.D.
-0.7
+0.3
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.8
+0.4
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
-0.6
+0.3
phage
JP1 MOI 10
N.D.
N.D.
N.D.
-0.6
+0.4
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
N.D.
N.D.
N.D.
-0.7
+0.6
phage
P. simiae ORA MOI 1
N.D.
N.D.
N.D.
+0.4
-0.5
rhizosphere
rhizosphere sample 1; outgrowth in LB
N.D.
N.D.
N.D.
-0.4
+0.5
agar plate interaction control
Parafilmed volatile agar plate with no fungus
N.D.
N.D.
N.D.
-0.4
+0.6
phage
P. simiae OR Antrim MOI 10
N.D.
N.D.
N.D.
+0.5
-0.3
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
N.D.
+0.6
-0.3
phage
P. simiae Grant Run MOI 0.1
N.D.
N.D.
N.D.
+0.7
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
N.D.
N.D.
N.D.
+0.7
-0.2
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
N.D.
N.D.
N.D.
+0.3
+0.6
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
N.D.
N.D.
N.D.
+0.7
+0.1
seeds
Growth on radish seeds for 72 hours
N.D.
N.D.
N.D.
+0.7
+0.3
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
N.D.
N.D.
N.D.
+0.4
+1.0
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