Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_25655 and PS417_25660 are separated by 165 nucleotidesPS417_25660 and PS417_25665 are separated by 136 nucleotidesPS417_25665 and PS417_25670 are separated by 24 nucleotidesPS417_25670 and PS417_25675 are separated by 70 nucleotides PS417_25655: PS417_25655 - iron--sulfur cluster insertion protein ErpA, at 5,542,382 to 5,542,732 _25655 PS417_25660: PS417_25660 - N-acetyl-gamma-glutamyl-phosphate reductase, at 5,542,898 to 5,543,932 _25660 PS417_25665: PS417_25665 - membrane protein, at 5,544,069 to 5,544,497 _25665 PS417_25670: PS417_25670 - 2-nitropropane dioxygenase, at 5,544,522 to 5,545,484 _25670 PS417_25675: PS417_25675 - membrane protein, at 5,545,555 to 5,546,511 _25675
Group Condition PS417_25655 PS417_25660 PS417_25665 PS417_25670 PS417_25675
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -1.3 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -1.2 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.8 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. N.D. N.D. -0.5 -0.6
soil soil sample 3; outgrowth in LB N.D. N.D. N.D. -0.9 -0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.4 -0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.0 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.5 -0.4
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB N.D. N.D. N.D. -0.4 -0.5
no stress control on plate growth on LB_noSalt plate N.D. N.D. N.D. -0.4 -0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. N.D. N.D. -0.5 -0.4
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.1 -0.8
phage JP1 MOI 1 N.D. N.D. N.D. -0.7 +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. +0.2 -0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. N.D. -0.7 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.8 +0.4
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. -0.6 +0.3
phage JP1 MOI 10 N.D. N.D. N.D. -0.6 +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. N.D. -0.7 +0.6
phage P. simiae ORA MOI 1 N.D. N.D. N.D. +0.4 -0.5
rhizosphere rhizosphere sample 1; outgrowth in LB N.D. N.D. N.D. -0.4 +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. N.D. -0.4 +0.6
phage P. simiae OR Antrim MOI 10 N.D. N.D. N.D. +0.5 -0.3
seeds Growth on radish seeds for 72 hours N.D. N.D. N.D. +0.6 -0.3
phage P. simiae Grant Run MOI 0.1 N.D. N.D. N.D. +0.7 -0.3
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. N.D. +0.7 -0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. N.D. N.D. +0.3 +0.6
supernatant LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x N.D. N.D. N.D. +0.7 +0.1
seeds Growth on radish seeds for 72 hours N.D. N.D. N.D. +0.7 +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. N.D. N.D. +0.4 +1.0
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