Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_25325 and PS417_25330 are separated by 171 nucleotidesPS417_25330 and PS417_25335 overlap by 4 nucleotidesPS417_25335 and PS417_25340 overlap by 11 nucleotidesPS417_25340 and PS417_25345 are separated by 4 nucleotides PS417_25325: PS417_25325 - mechanosensitive ion channel protein, at 5,482,683 to 5,484,767 _25325 PS417_25330: PS417_25330 - hypothetical protein, at 5,484,939 to 5,485,718 _25330 PS417_25335: PS417_25335 - cardiolipin synthase 2, at 5,485,715 to 5,486,977 _25335 PS417_25340: PS417_25340 - membrane protein, at 5,486,967 to 5,487,914 _25340 PS417_25345: PS417_25345 - permease, at 5,487,919 to 5,488,674 _25345
Group Condition PS417_25325 PS417_25330 PS417_25335 PS417_25340 PS417_25345
phage P. simiae ORA MOI 0.1 +0.1 +0.1 -0.1 -1.4 N.D.
phage JP1 MOI 1 +0.0 +0.2 -0.1 -1.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 -0.2 +0.0 -0.4 N.D.
soil soil sample 7; outgrowth in LB -0.1 -0.5 -0.1 -0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.3 +0.2 -0.3 -0.7 N.D.
phage Wcs_1 -0.2 -0.2 -0.1 -0.4 N.D.
solid stress Fraxetin 2 mM; solid stress -0.3 -0.1 +0.0 -0.7 N.D.
seeds Growth on radish seeds for 72 hours -0.3 -0.3 +0.2 -0.6 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.8 +0.3 +0.0 -0.5 N.D.
nophagecontrol Only library -0.2 -0.3 +0.1 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 -0.5 -0.2 -0.1 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.2 -0.2 +0.2 -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.1 +0.6 -0.2 -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.7 -0.2 -0.1 +0.5 N.D.
phage P. simiae OR Antrim MOI 0.1 -0.8 +0.3 -0.0 +0.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.6 -0.2 +0.1 +0.4 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 -0.4 +0.3 -0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 -0.6 -0.1 +0.5 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 +0.1 +0.1 -0.6 N.D.
seeds Growth on radish seeds for 72 hours -0.7 +0.0 -0.1 +0.9 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.2 -0.3 -0.2 +0.4 N.D.
stress Gentamicin 0.008 mg/ml -0.3 +0.4 +0.2 +0.3 N.D.
stress Doxycycline hyclate 0.001 mg/ml -0.1 +0.2 +0.2 +0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=casaminos; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.4 -0.2 +0.0 +0.5 N.D.
carbon source 2-Deoxy-D-Ribose 10 mM (C) +0.4 -0.2 +0.2 +0.5 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.4 -0.2 -0.0 +0.8 N.D.
root root sample 6; outgrowth in LB +0.9 -0.1 +0.1 +0.2 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.2 +0.2 +0.1 +0.7 N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +0.3 -0.0 -0.0 +1.1 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.4 +0.4 -0.1 +1.2 N.D.
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