Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_25230 and PS417_25235 are separated by 150 nucleotidesPS417_25235 and PS417_25240 are separated by 66 nucleotidesPS417_25240 and PS417_25245 are separated by 18 nucleotidesPS417_25245 and PS417_25250 overlap by 4 nucleotides PS417_25230: PS417_25230 - GTP cyclohydrolase, at 5,467,847 to 5,468,464 _25230 PS417_25235: PS417_25235 - ABC transporter substrate-binding protein, at 5,468,615 to 5,469,334 _25235 PS417_25240: PS417_25240 - thiamine monophosphate kinase, at 5,469,401 to 5,470,366 _25240 PS417_25245: PS417_25245 - antitermination protein NusB, at 5,470,385 to 5,470,885 _25245 PS417_25250: PS417_25250 - 6,7-dimethyl-8-ribityllumazine synthase, at 5,470,882 to 5,471,358 _25250
Group Condition PS417_25230 PS417_25235 PS417_25240 PS417_25245 PS417_25250
carbon source Xylitol (C) N.D. -0.4 N.D. N.D. N.D.
root root sample 6; outgrowth in LB N.D. -0.4 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. -0.4 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 N.D. N.D. N.D.
phage JP1 MOI 1 N.D. -0.3 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days N.D. -0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.3 N.D. N.D. N.D.
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. -0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. -0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. -0.3 N.D. N.D. N.D.
stress Gentamicin 0.008 mg/ml N.D. -0.3 N.D. N.D. N.D.
phage Wcs_1 N.D. -0.3 N.D. N.D. N.D.
phage P. simiae ORA MOI 10 N.D. +0.3 N.D. N.D. N.D.
phage P. simiae Grant Run MOI 0.1 N.D. +0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. +0.3 N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. +0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.3 N.D. N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 N.D. +0.3 N.D. N.D. N.D.
soil soil sample 7; outgrowth in LB N.D. +0.3 N.D. N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X N.D. +0.3 N.D. N.D. N.D.
phage JP1 MOI 10 N.D. +0.3 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant N.D. +0.4 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant N.D. +0.4 N.D. N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=Fusarium; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. +0.4 N.D. N.D. N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days N.D. +0.4 N.D. N.D. N.D.
phage P. simiae ORA MOI 0.1 N.D. +0.4 N.D. N.D. N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml N.D. +0.4 N.D. N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant N.D. +0.4 N.D. N.D. N.D.
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