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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_25210 and PS417_25215 are separated by 93 nucleotides
PS417_25215 and PS417_25220 are separated by 43 nucleotides
PS417_25220 and PS417_25225 overlap by 4 nucleotides
PS417_25225 and PS417_25230 overlap by 4 nucleotides
PS417_25210: PS417_25210 - TonB-dependent receptor, at 5,464,407 to 5,466,302
_25210
PS417_25215: PS417_25215 - hypothetical protein, at 5,466,396 to 5,466,602
_25215
PS417_25220: PS417_25220 - iron-hydroxamate ABC transporter substrate-binding protein, at 5,466,646 to 5,467,434
_25220
PS417_25225: PS417_25225 - MFS transporter, at 5,467,431 to 5,467,850
_25225
PS417_25230: PS417_25230 - GTP cyclohydrolase, at 5,467,847 to 5,468,464
_25230
Group
Condition
PS417
_25210
PS417
_25215
PS417
_25220
PS417
_25225
PS417
_25230
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
N.D.
-0.4
-3.0
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
N.D.
-0.3
-2.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
N.D.
-0.9
-0.9
N.D.
nitrogen source
Adenine (N)
-0.5
N.D.
-1.2
-0.4
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.1
N.D.
-1.3
-0.8
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
N.D.
+0.3
-2.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
N.D.
-0.2
-1.8
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
N.D.
+0.1
-2.1
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
+0.1
N.D.
-0.0
-2.0
N.D.
seeds
Growth on radish seeds for 72 hours
+0.1
N.D.
+0.6
-2.7
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.1
N.D.
+0.1
-1.8
N.D.
stress
Phosphomycin 0.003 mg/ml
-0.0
N.D.
-0.7
-1.0
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
+0.0
N.D.
-0.3
-1.5
N.D.
phage
P. simiae Grant Run MOI 0.1
-0.3
N.D.
-0.2
-1.2
N.D.
soil
soil sample 6; outgrowth in LB
-0.2
N.D.
+0.2
-1.5
N.D.
phage
JP1 MOI 1
+0.1
N.D.
-0.3
-1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
N.D.
+0.1
-1.4
N.D.
seeds
Growth on radish seeds for 72 hours
-0.1
N.D.
+0.4
-1.4
N.D.
carbon source
L-Valine (C)
-0.1
N.D.
+0.7
-1.5
N.D.
supernatant control
Vogels_fungal_media 0.1X
+0.3
N.D.
-1.5
+0.4
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.1
N.D.
+0.7
-1.4
N.D.
phage
P. simiae ORA MOI 1
+0.4
N.D.
+0.5
-1.1
N.D.
phage
JP1 MOI 10
+0.5
N.D.
-1.0
+0.7
N.D.
phage
JP1 MOI 1
-0.0
N.D.
-0.6
+1.1
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.4
N.D.
+0.5
+0.8
N.D.
no phage control
Time6-nophage
-0.2
N.D.
+0.3
+1.2
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
-0.0
+0.9
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.0
N.D.
+0.3
+1.4
N.D.
carbon source
L-Valine (C)
+0.5
N.D.
-0.6
+1.9
N.D.
soil
soil sample 7; outgrowth in LB
-0.1
N.D.
+0.5
+1.5
N.D.
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