Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_25200 and PS417_25205 are separated by 110 nucleotidesPS417_25205 and PS417_25210 are separated by 156 nucleotidesPS417_25210 and PS417_25215 are separated by 93 nucleotidesPS417_25215 and PS417_25220 are separated by 43 nucleotides PS417_25200: PS417_25200 - geranyl transferase, at 5,461,354 to 5,462,241 _25200 PS417_25205: PS417_25205 - 1-deoxy-D-xylulose-5-phosphate synthase, at 5,462,352 to 5,464,250 _25205 PS417_25210: PS417_25210 - TonB-dependent receptor, at 5,464,407 to 5,466,302 _25210 PS417_25215: PS417_25215 - hypothetical protein, at 5,466,396 to 5,466,602 _25215 PS417_25220: PS417_25220 - iron-hydroxamate ABC transporter substrate-binding protein, at 5,466,646 to 5,467,434 _25220
Group Condition PS417_25200 PS417_25205 PS417_25210 PS417_25215 PS417_25220
nitrogen source Adenine (N) N.D. N.D. -0.5 N.D. -1.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI N.D. N.D. +0.1 N.D. -1.3
supernatant control Vogels_fungal_media 0.1X N.D. N.D. +0.3 N.D. -1.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. -0.2 N.D. -0.9
carbon source Carbon source D-Mannitol 2.5 mM N.D. N.D. -0.2 N.D. -0.6
stress Phosphomycin 0.003 mg/ml N.D. N.D. -0.0 N.D. -0.7
exudates RCH2_defined_glucose with Brachypodium distachyon 13-day exudates 1x N.D. N.D. -0.1 N.D. -0.6
solid stress Fraxetin 3 mM; solid stress N.D. N.D. -0.1 N.D. -0.5
seeds Growth on radish seeds for 24 hours N.D. N.D. +0.1 N.D. -0.7
phage JP1 MOI 10 N.D. N.D. +0.5 N.D. -1.0
stress Lomefloxacin 0.0001 mM N.D. N.D. +0.1 N.D. -0.6
phage P. simiae Grant Run MOI 0.1 N.D. N.D. +0.2 N.D. -0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.2 N.D. -0.5
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X N.D. N.D. +0.3 N.D. -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.3 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.003 mg/ml N.D. N.D. +0.4 N.D. -0.5
carbon source L-Valine (C) N.D. N.D. +0.5 N.D. -0.6
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. -0.4 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.4 N.D. -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus N.D. N.D. +0.5 N.D. -0.2
seeds Growth on radish seeds for 72 hours N.D. N.D. -0.3 N.D. +0.7
soil soil sample 5; outgrowth in LB N.D. N.D. -0.1 N.D. +0.7
carbon source L-Valine (C) N.D. N.D. -0.1 N.D. +0.7
soil soil sample 8; outgrowth in LB N.D. N.D. +0.3 N.D. +0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.2 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days N.D. N.D. +0.1 N.D. +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 N.D. N.D. +0.1 N.D. +0.7
seeds Growth on radish seeds for 72 hours N.D. N.D. +0.1 N.D. +0.6
phage P. simiae ORA MOI 1 N.D. N.D. +0.4 N.D. +0.5
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days N.D. N.D. +0.3 N.D. +0.7
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PS417_25200
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PS417_25205
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PS417_25215
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