Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_25030 and PS417_25035 are separated by 105 nucleotidesPS417_25035 and PS417_25040 are separated by 834 nucleotidesPS417_25040 and PS417_25045 are separated by 14 nucleotidesPS417_25045 and PS417_25055 are separated by 717 nucleotides PS417_25030: PS417_25030 - hypothetical protein, at 5,428,826 to 5,429,038 _25030 PS417_25035: PS417_25035 - hypothetical protein, at 5,429,144 to 5,430,181 _25035 PS417_25040: PS417_25040 - hypothetical protein, at 5,431,016 to 5,431,270 _25040 PS417_25045: PS417_25045 - hypothetical protein, at 5,431,285 to 5,431,584 _25045 PS417_25055: PS417_25055 - transposase, at 5,432,302 to 5,432,499 _25055
Group Condition PS417_25030 PS417_25035 PS417_25040 PS417_25045 PS417_25055
solid stress 80% Methanol 0.5 mM; solid stress -0.7 -0.1 -0.3 -0.3 -0.6
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Glutamine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.8 -0.2 +0.3 -0.3 -0.6
carbon source 2'-Deoxyinosine 5 mM (C) -0.9 +0.1 -0.2 +0.3 -0.5
phage P. simiae OR Antrim MOI 0.1 -0.8 -0.2 -0.1 +0.4 -0.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.7 +0.2 -0.3 -0.5 +0.3
agar plate interaction control Taped volatile agar plate with no fungus -0.8 +0.1 -0.3 +0.4 -0.3
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.4 +0.4 +0.2 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +1.0 -0.4 -0.3 -0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -0.6 +0.3 +0.4 -0.3 -0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 -0.4 +0.0 +0.3 -0.9
phage P. simiae OR Antrim MOI 0.1 -0.9 +0.1 -0.3 +0.2 +0.6
supernatant control Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB -1.0 +0.3 +0.1 +0.3 +0.2
motility outer cut, LB soft agar motility assay -1.1 +0.3 +0.2 +0.2 +0.4
seeds Growth on radish seeds for 72 hours +0.9 -0.0 -0.1 -0.2 -0.7
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.9 +0.5 -0.1 +1.1 -0.4
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 1X -0.9 +0.4 +0.1 +0.4 +0.2
phage JP1 MOI 1 -0.8 +0.4 -0.0 +0.2 +0.4
supernatant control Vogels_fungal_media; pH 5.5 -0.7 +0.2 +0.0 +0.3 +0.6
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, non-filtered; 0.5X; growth supplemented with 0.4X LB -0.7 +0.4 +0.1 +0.4 +0.4
phage JP1 MOI 1 +0.6 -0.1 -0.7 +0.6 +0.2
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.1 +0.1 +0.4 -0.1 -0.9
motility outer cut, LB soft agar motility assay -0.6 +0.2 +0.3 +0.2 +0.7
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB -0.7 +0.3 +0.4 +0.1 +0.7
supernatant control Vogels_fungal_media; pH 5.5 -0.4 +0.5 +0.2 +0.7 +0.4
nophagecontrol Only library +0.7 +0.3 -0.4 +0.2 +0.5
soil soil sample 2; outgrowth in LB +0.9 +0.3 +0.0 -0.1 +0.6
stress LB_noSalt with Chloride 1100 mM +0.2 +0.5 +0.5 +0.4 +0.2
rhizosphere rhizosphere sample 8; outgrowth in LB +0.3 +0.1 +0.6 -0.1 +0.9
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days +1.5 +0.2 -0.0 -0.0 +0.3
phage P. simiae Grant Run MOI 10 +0.9 +0.1 +0.3 +0.4 +0.3
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