Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_24955 and PS417_24960 are separated by 112 nucleotidesPS417_24960 and PS417_24965 are separated by 81 nucleotidesPS417_24965 and PS417_24985 are separated by 4797 nucleotidesPS417_24985 and PS417_24990 are separated by 448 nucleotides PS417_24955: PS417_24955 - hypothetical protein, at 5,411,756 to 5,411,992 _24955 PS417_24960: PS417_24960 - transposase, at 5,412,105 to 5,413,682 _24960 PS417_24965: PS417_24965 - transposase, at 5,413,764 to 5,414,099 _24965 PS417_24985: PS417_24985 - hypothetical protein, at 5,418,897 to 5,419,106 _24985 PS417_24990: PS417_24990 - exotoxin, at 5,419,555 to 5,420,103 _24990
Group Condition PS417_24955 PS417_24960 PS417_24965 PS417_24985 PS417_24990
solid stress Fraxetin 3 mM; solid stress +0.1 -0.2 N.D. -0.2 -0.9
nophagecontrol Only library -0.0 -0.0 N.D. -0.4 -0.8
liquid stress Fraxetin 1 mM; liquid stress -0.0 -0.1 N.D. +0.1 -1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 +0.1 N.D. +0.1 -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.1 N.D. +0.2 -0.9
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.5 -0.1 N.D. +0.3 -0.3
phage JP1 MOI 1 +0.2 +0.2 N.D. -0.5 -0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.3 +0.1 N.D. -0.3 -0.5
phage P. simiae OR Antrim MOI 10 +0.1 -0.1 N.D. -0.7 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI -0.7 +0.1 N.D. +0.2 +0.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.2 +0.1 N.D. +0.2 -0.7
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.5 +0.2 N.D. +0.2 -0.2
phage JP1 MOI 10 -0.1 -0.2 N.D. -0.4 +0.5
rhizosphere rhizosphere sample 2; outgrowth in LB +0.1 -0.4 N.D. +0.4 +0.3
phage P. simiae Grant Run MOI 0.1 +0.2 -0.1 N.D. -0.2 +0.6
phage FRSphage -0.2 -0.1 N.D. +0.1 +1.0
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.0 -0.1 N.D. +1.0 -0.0
carbon source L-Valine (C) +0.1 -0.2 N.D. +0.3 +0.6
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.5 +0.1 N.D. +0.4 -0.1
soil soil sample 7; outgrowth in LB -0.0 -0.1 N.D. +0.2 +0.9
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.3 -0.1 N.D. +0.4 +0.6
stress methylglyoxal 0.032 vol% +0.2 +0.1 N.D. +0.5 +0.3
phage JP1 MOI 0.1 +0.1 -0.0 N.D. +0.8 +0.3
motility outer cut, LB soft agar motility assay +0.2 +0.1 N.D. +0.2 +0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 +0.0 N.D. +0.0 +0.5
root root sample 6; outgrowth in LB +0.1 +0.2 N.D. +0.5 +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.4 N.D. +0.4 +0.3
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.3 -0.0 N.D. +0.2 +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.2 +0.2 N.D. +0.8 +0.3
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.7 +0.4 N.D. +0.3 +0.5
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PS417_24960
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