Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_24950 and PS417_24955 are separated by 653 nucleotidesPS417_24955 and PS417_24960 are separated by 112 nucleotidesPS417_24960 and PS417_24965 are separated by 81 nucleotidesPS417_24965 and PS417_24985 are separated by 4797 nucleotides PS417_24950: PS417_24950 - hypothetical protein, at 5,407,491 to 5,411,102 _24950 PS417_24955: PS417_24955 - hypothetical protein, at 5,411,756 to 5,411,992 _24955 PS417_24960: PS417_24960 - transposase, at 5,412,105 to 5,413,682 _24960 PS417_24965: PS417_24965 - transposase, at 5,413,764 to 5,414,099 _24965 PS417_24985: PS417_24985 - hypothetical protein, at 5,418,897 to 5,419,106 _24985
Group Condition PS417_24950 PS417_24955 PS417_24960 PS417_24965 PS417_24985
phage P. simiae OR Antrim MOI 10 +0.1 +0.1 -0.1 N.D. -0.7
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.1 -0.5 +0.1 N.D. +0.1
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.2 -0.7 +0.1 N.D. +0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.5 -0.1 N.D. +0.3
phage JP1 MOI 1 +0.0 +0.2 +0.2 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +0.1 -0.5 +0.2 N.D. +0.2
in planta Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days +0.2 +0.2 +0.1 N.D. -0.5
stress Gentamicin 0.008 mg/ml -0.0 +0.6 -0.1 N.D. -0.4
rhizosphere rhizosphere sample 2; outgrowth in LB +0.0 +0.1 -0.4 N.D. +0.4
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 -0.2 -0.1 N.D. +0.5
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.0 +0.3 -0.2 N.D. +0.4
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI +0.1 +0.3 -0.1 N.D. +0.4
phage P. simiae Grant Run MOI 0.1 +0.2 +0.6 -0.1 N.D. +0.1
phage P. simiae OR Antrim MOI 1 +0.2 +0.3 -0.0 N.D. +0.4
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.3 +0.2 +0.2 N.D. +0.2
root root sample 6; outgrowth in LB +0.0 +0.1 +0.2 N.D. +0.5
soil soil sample 2; outgrowth in LB +0.2 +0.5 -0.0 N.D. +0.2
motility outer cut, LB soft agar motility assay +0.2 +0.2 +0.3 N.D. +0.1
phage JP1 MOI 0.1 +0.0 +0.1 -0.0 N.D. +0.8
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.4 +0.1 N.D. +0.3
motility inner cut, LB soft agar motility assay +0.3 +0.1 +0.3 N.D. +0.2
soil soil sample 4; outgrowth in LB +0.2 +0.5 +0.0 N.D. +0.3
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +0.1 -0.0 -0.1 N.D. +1.0
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 +0.5 +0.2 N.D. +0.2
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant +0.1 +0.1 +0.4 N.D. +0.4
stress methylglyoxal 0.032 vol% +0.3 +0.2 +0.1 N.D. +0.5
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant +0.2 +0.5 +0.1 N.D. +0.4
stress LB_noSalt with Chloride 1100 mM +0.4 +0.2 +0.2 N.D. +0.5
agar plate interaction control Parafilmed volatile agar plate with no fungus +0.1 +0.2 +0.2 N.D. +0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 +0.7 +0.4 N.D. +0.3
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