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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_24715 and PS417_24720 are separated by 17 nucleotides
PS417_24720 and PS417_24725 overlap by 11 nucleotides
PS417_24725 and PS417_24730 are separated by 15 nucleotides
PS417_24730 and PS417_24735 are separated by 9 nucleotides
PS417_24715: PS417_24715 - lytic transglycosylase, at 5,362,045 to 5,363,055
_24715
PS417_24720: PS417_24720 - rod shape-determining protein RodA, at 5,363,073 to 5,364,218
_24720
PS417_24725: PS417_24725 - penicillin-binding protein 2, at 5,364,208 to 5,366,106
_24725
PS417_24730: PS417_24730 - 50S rRNA methyltransferase, at 5,366,122 to 5,366,589
_24730
PS417_24735: PS417_24735 - ribosome-associated protein IOJAP, at 5,366,599 to 5,367,093
_24735
Group
Condition
PS417
_24715
PS417
_24720
PS417
_24725
PS417
_24730
PS417
_24735
stress
Neomycin 0.04 mg/ml
-1.8
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.0
N.D.
N.D.
N.D.
N.D.
stress
L-Lysine 60 mM
-0.9
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.8
N.D.
N.D.
N.D.
N.D.
stress
Polymyxin B sulfate 0.006 mg/ml
-0.7
N.D.
N.D.
N.D.
N.D.
carbon source
L-Valine (C)
-0.7
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.7
N.D.
N.D.
N.D.
N.D.
agar plate interaction control
Taped volatile agar plate with no fungus
-0.7
N.D.
N.D.
N.D.
N.D.
phage
P. simiae ORA MOI 1
-0.6
N.D.
N.D.
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.6
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.6
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.6
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.6
N.D.
N.D.
N.D.
N.D.
solid stress
Fraxetin 3 mM; solid stress
+0.7
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
N.D.
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 10
+0.7
N.D.
N.D.
N.D.
N.D.
solid stress
Fraxetin 3 mM; solid stress
+0.7
N.D.
N.D.
N.D.
N.D.
phage
P. simiae Grant Run MOI 0.1
+0.7
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
N.D.
N.D.
N.D.
N.D.
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.7
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.7
N.D.
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 0.1
+0.8
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.8
N.D.
N.D.
N.D.
N.D.
phage
JP1 MOI 1
+0.8
N.D.
N.D.
N.D.
N.D.
phage
P. simiae OR Antrim MOI 10
+0.9
N.D.
N.D.
N.D.
N.D.
stress
1-ethyl-3-methylimidazolium acetate 35 mM
+0.9
N.D.
N.D.
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Asparagine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.9
N.D.
N.D.
N.D.
N.D.
phage
JP1 MOI 0.1
+1.0
N.D.
N.D.
N.D.
N.D.
stress
1-ethyl-3-methylimidazolium chloride 200 mM
+1.0
N.D.
N.D.
N.D.
N.D.
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