Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_24620 and PS417_24625 are separated by 131 nucleotidesPS417_24625 and PS417_24630 are separated by 230 nucleotidesPS417_24630 and PS417_24635 overlap by 8 nucleotidesPS417_24635 and PS417_24640 are separated by 41 nucleotides PS417_24620: PS417_24620 - hypothetical protein, at 5,342,851 to 5,343,177 _24620 PS417_24625: PS417_24625 - (dimethylallyl)adenosine tRNA methylthiotransferase, at 5,343,309 to 5,344,637 _24625 PS417_24630: PS417_24630 - ATP-binding protein, at 5,344,868 to 5,345,872 _24630 PS417_24635: PS417_24635 - rRNA maturation factor, at 5,345,865 to 5,346,323 _24635 PS417_24640: PS417_24640 - magnesium transporter, at 5,346,365 to 5,347,204 _24640
Group Condition PS417_24620 PS417_24625 PS417_24630 PS417_24635 PS417_24640
motility inner cut, LB soft agar motility assay -2.2 -0.7 -0.3 N.D. N.D.
soil soil sample 3; outgrowth in LB -1.3 -0.9 -0.9 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.7 -0.4 -0.8 N.D. N.D.
stress methylglyoxal 0.032 vol% -1.0 -0.7 -1.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.2 -0.3 -0.2 N.D. N.D.
motility inner cut, LB soft agar motility assay -1.4 -0.4 -0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.0 -0.1 -0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 -0.3 -0.4 N.D. N.D.
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -0.7 -0.9 -0.4 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant -1.1 -0.6 -0.2 N.D. N.D.
stress Cisplatin 0.01 mg/ml +0.4 +0.1 -2.3 N.D. N.D.
solid stress Fraxetin 3 mM; solid stress -1.0 -0.3 -0.5 N.D. N.D.
seeds Growth on radish seeds for 72 hours -0.8 -0.1 -0.9 N.D. N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -1.3 -0.6 +0.1 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 -1.5 +0.3 -0.5 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.5 -0.4 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.5 -1.0 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.4 -0.4 +1.1 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.3 +0.2 +0.4 N.D. N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml -1.2 +0.1 +0.6 N.D. N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.1 +0.0 +0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.7 +0.8 -0.3 N.D. N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant -1.0 -0.6 +1.6 N.D. N.D.
stress Gentamicin 0.008 mg/ml +0.3 +0.9 -1.1 N.D. N.D.
nophagecontrol Only library -1.2 +0.2 +1.2 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days +0.5 -0.9 +0.7 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB +1.1 -0.1 -0.6 N.D. N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -1.1 +0.4 +1.2 N.D. N.D.
seeds Growth on radish seeds for 72 hours +1.5 -0.2 +0.3 N.D. N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X +1.3 -0.0 +0.6 N.D. N.D.
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