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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_24620 and PS417_24625 are separated by 131 nucleotides
PS417_24625 and PS417_24630 are separated by 230 nucleotides
PS417_24630 and PS417_24635 overlap by 8 nucleotides
PS417_24635 and PS417_24640 are separated by 41 nucleotides
PS417_24620: PS417_24620 - hypothetical protein, at 5,342,851 to 5,343,177
_24620
PS417_24625: PS417_24625 - (dimethylallyl)adenosine tRNA methylthiotransferase, at 5,343,309 to 5,344,637
_24625
PS417_24630: PS417_24630 - ATP-binding protein, at 5,344,868 to 5,345,872
_24630
PS417_24635: PS417_24635 - rRNA maturation factor, at 5,345,865 to 5,346,323
_24635
PS417_24640: PS417_24640 - magnesium transporter, at 5,346,365 to 5,347,204
_24640
Group
Condition
PS417
_24620
PS417
_24625
PS417
_24630
PS417
_24635
PS417
_24640
motility
inner cut, LB soft agar motility assay
-2.2
-0.7
-0.3
N.D.
N.D.
soil
soil sample 3; outgrowth in LB
-1.3
-0.9
-0.9
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.7
-0.4
-0.8
N.D.
N.D.
stress
methylglyoxal 0.032 vol%
-1.0
-0.7
-1.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.2
-0.3
-0.2
N.D.
N.D.
motility
inner cut, LB soft agar motility assay
-1.4
-0.4
-0.5
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-2.0
-0.1
-0.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.3
-0.3
-0.4
N.D.
N.D.
in planta
Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
-0.7
-0.9
-0.4
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI ago3mutant
-1.1
-0.6
-0.2
N.D.
N.D.
stress
Cisplatin 0.01 mg/ml
+0.4
+0.1
-2.3
N.D.
N.D.
solid stress
Fraxetin 3 mM; solid stress
-1.0
-0.3
-0.5
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
-0.8
-0.1
-0.9
N.D.
N.D.
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-1.3
-0.6
+0.1
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Isoleucine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-1.5
+0.3
-0.5
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Tryptophan; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.5
-0.4
+0.6
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.3
-0.5
-1.0
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.4
-0.4
+1.1
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.3
+0.2
+0.4
N.D.
N.D.
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-1.2
+0.1
+0.6
N.D.
N.D.
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
+0.0
+0.6
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
+0.8
-0.3
N.D.
N.D.
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-1.0
-0.6
+1.6
N.D.
N.D.
stress
Gentamicin 0.008 mg/ml
+0.3
+0.9
-1.1
N.D.
N.D.
nophagecontrol
Only library
-1.2
+0.2
+1.2
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=20h_outgrowth_LB; Time=7_days
+0.5
-0.9
+0.7
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.8X; growth supplemented with 0.4X LB
+1.1
-0.1
-0.6
N.D.
N.D.
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.1
+0.4
+1.2
N.D.
N.D.
seeds
Growth on radish seeds for 72 hours
+1.5
-0.2
+0.3
N.D.
N.D.
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+1.3
-0.0
+0.6
N.D.
N.D.
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