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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_24390 and PS417_24395 overlap by 4 nucleotides
PS417_24395 and PS417_24400 overlap by 4 nucleotides
PS417_24400 and PS417_24405 are separated by 162 nucleotides
PS417_24405 and PS417_24410 are separated by 81 nucleotides
PS417_24390: PS417_24390 - peptidase, at 5,294,093 to 5,295,409
_24390
PS417_24395: PS417_24395 - hemolysin D, at 5,295,406 to 5,296,731
_24395
PS417_24400: PS417_24400 - peptidase, at 5,296,728 to 5,298,464
_24400
PS417_24405: PS417_24405 - heme acquisition protein HasAp, at 5,298,627 to 5,299,247
_24405
PS417_24410: PS417_24410 - ligand-gated channel, at 5,299,329 to 5,301,986
_24410
Group
Condition
PS417
_24390
PS417
_24395
PS417
_24400
PS417
_24405
PS417
_24410
motility
inner cut, LB soft agar motility assay
-0.5
-0.2
-0.5
-0.5
-0.0
phage
P. simiae OR1 MOI 0.1
-0.1
+0.1
-1.2
-0.4
+0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.2
-0.2
-0.4
-0.7
-0.0
motility
inner cut, LB soft agar motility assay
-0.3
-0.4
-0.6
-0.2
-0.0
stress
Gentamicin 0.008 mg/ml
-0.3
-1.2
+0.4
-0.2
-0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.3
-0.1
-0.8
-0.2
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Valine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
-0.2
-0.5
-0.5
-0.0
supernatant control
Vogels_fungal_media 0.1X
-0.4
+0.5
-1.0
-0.6
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
-0.4
-0.9
+0.1
-0.0
agar plate interaction control
Parafilmed volatile agar plate with no fungus
-0.0
-1.3
+0.5
-0.3
-0.2
supernatant control
Vogels_fungal_media 0.1X
+0.2
+0.0
-0.9
-0.3
+0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-1.1
-0.5
+0.2
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.0
-0.5
+0.5
-0.8
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.2
+0.5
-0.7
-0.2
-0.1
seeds
Growth on radish seeds for 72 hours
-0.1
-0.6
-0.4
+0.4
+0.0
agar plate interaction control
Taped volatile agar plate with no fungus
-0.5
-0.2
+0.4
-0.1
-0.1
carbon source
L-Valine (C)
+0.3
-0.1
-0.3
-0.6
+0.3
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
+0.0
+0.6
-0.6
-0.2
-0.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
+0.3
-0.5
-0.3
+0.2
carbon source
Carbon source L-Citrulline 10 mM
+0.2
-0.5
+0.1
+0.4
-0.1
stress
Choline chloride 300 mM
+0.3
-0.2
+0.5
-0.3
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.0
-0.1
+0.2
+0.6
-0.4
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.3
+0.7
+0.5
-0.5
+0.1
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
-0.3
+0.8
+0.1
+0.2
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI tmk3mutant
-0.0
+1.0
-0.1
-0.4
+0.1
carbon source
Deoxyribonucleic from herring sperm 10 mg/mL (C)
-0.1
+0.8
-0.3
+0.1
+0.2
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.1
+1.2
+0.1
-0.0
-0.2
supernatant
RCH2_defined_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.25x
-0.0
+0.4
+0.5
+0.5
-0.1
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
+0.3
+0.3
-0.2
+1.0
+0.1
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
+0.0
+0.2
+1.1
+0.2
+0.3
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