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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_24380 and PS417_24385 overlap by 4 nucleotides
PS417_24385 and PS417_24390 are separated by 97 nucleotides
PS417_24390 and PS417_24395 overlap by 4 nucleotides
PS417_24395 and PS417_24400 overlap by 4 nucleotides
PS417_24380: PS417_24380 - guanine permease, at 5,291,624 to 5,292,919
_24380
PS417_24385: PS417_24385 - tRNA (uracil-5-)-methyltransferase, at 5,292,916 to 5,293,995
_24385
PS417_24390: PS417_24390 - peptidase, at 5,294,093 to 5,295,409
_24390
PS417_24395: PS417_24395 - hemolysin D, at 5,295,406 to 5,296,731
_24395
PS417_24400: PS417_24400 - peptidase, at 5,296,728 to 5,298,464
_24400
Group
Condition
PS417
_24380
PS417
_24385
PS417
_24390
PS417
_24395
PS417
_24400
stress
Gentamicin 0.008 mg/ml
-1.0
-0.7
-0.3
-1.2
+0.4
phage
P. simiae OR1 MOI 0.1
-0.9
-0.7
-0.1
+0.1
-1.2
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.8
-0.6
-0.3
-0.1
-0.8
motility
inner cut, LB soft agar motility assay
-0.4
-0.8
-0.5
-0.2
-0.5
motility
inner cut, LB soft agar motility assay
-0.4
-0.4
-0.3
-0.4
-0.6
root
root sample 6; outgrowth in LB
-1.6
-0.4
+0.3
-0.2
-0.0
supernatant control
Vogels_fungal_media 0.1X
-0.7
-0.5
-0.4
+0.5
-1.0
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
-0.1
-0.5
-0.1
-0.4
-0.9
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-0.4
+0.2
-1.1
-0.5
liquid stress
Fraxetin 3 mM; liquid stress
-0.5
-1.5
+0.1
+0.0
+0.1
liquid stress
Fraxetin 3 mM; liquid stress
-0.4
-1.8
+0.1
+0.2
+0.1
liquid stress
Fraxetin 3 mM; liquid stress
-0.6
-1.5
-0.0
+0.4
-0.1
liquid stress
Scopoletin 3 mM; liquid stress
-0.6
-1.4
-0.1
+0.1
+0.4
supernatant control
Vogels_fungal_media 0.1X
-0.5
-0.3
+0.2
+0.0
-0.9
seeds
Growth on radish seeds for 72 hours
+0.3
-0.7
-0.1
-0.6
-0.4
liquid stress
Fraxetin 3 mM; liquid stress
-0.5
-1.2
+0.1
+0.0
+0.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-1.1
-0.8
+0.2
+0.7
-0.1
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
+0.2
-1.5
+0.1
-0.1
+0.4
seeds
Growth on radish seeds for 72 hours
-0.8
-0.3
-0.3
+0.5
+0.0
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-1.0
-0.7
-0.3
+0.7
+0.5
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50
-0.2
-1.0
-0.0
+0.7
-0.1
agar plate interaction control
Parafilmed volatile agar plate with no fungus
+0.4
-0.3
-0.0
-1.3
+0.5
supernatant
LB 0.5x with Supernatant; Trichoderma harzanium TH1 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.5
-0.8
+0.1
+0.4
+0.2
supernatant control
Vogels_fungal_media 0.5X; growth supplemented with 0.4X LB
-0.3
-0.3
+0.0
+0.6
-0.6
liquid stress
80% Methanol 3 mM; liquid stress
-0.3
-0.7
-0.0
+0.5
+0.5
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.7
-0.6
-0.1
+1.2
+0.1
supernatant
Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X
+1.1
-0.2
+0.1
-0.3
-0.3
agar plate interaction
Parafilmed volatile agar plate with Trichoderma atroviridae IMI
-0.3
-0.5
+0.0
+0.2
+1.1
in planta
Plant=Zea_mays-bx1; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days
+0.2
-0.7
+0.3
-0.0
+0.6
agar plate interaction control
Taped volatile agar plate with no fungus
+0.6
+0.4
-0.5
-0.2
+0.4
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