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Fitness for 5 genes in
Pseudomonas simiae WCS417
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500 nt
PS417_24210 and PS417_24215 are separated by 15 nucleotides
PS417_24215 and PS417_24220 are separated by 170 nucleotides
PS417_24220 and PS417_24225 are separated by 3 nucleotides
PS417_24225 and PS417_24230 are separated by 178 nucleotides
PS417_24210: PS417_24210 - dolichyl-phosphate-mannose-protein mannosyltransferase, at 5,250,649 to 5,252,082
_24210
PS417_24215: PS417_24215 - hypothetical protein, at 5,252,098 to 5,252,586
_24215
PS417_24220: PS417_24220 - hypothetical protein, at 5,252,757 to 5,254,577
_24220
PS417_24225: PS417_24225 - hypothetical protein, at 5,254,581 to 5,254,889
_24225
PS417_24230: PS417_24230 - ATP-dependent protease, at 5,255,068 to 5,257,506
_24230
Group
Condition
PS417
_24210
PS417
_24215
PS417
_24220
PS417
_24225
PS417
_24230
stress
methylglyoxal 0.032 vol%
+0.2
N.D.
-0.2
+0.4
-2.6
motility
inner cut, LB soft agar motility assay
-0.1
N.D.
-0.0
-0.7
-1.0
supernatant control
Vogels_fungal_media 0.1X
-0.4
N.D.
+0.0
-1.4
+0.1
motility
outer cut, LB soft agar motility assay
-0.0
N.D.
-0.1
+0.0
-1.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
N.D.
+0.2
-0.4
-1.3
motility_chemotaxis
Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50
+0.0
N.D.
+0.1
-0.8
-0.9
motility
inner cut, LB soft agar motility assay
-0.0
N.D.
+0.3
-0.7
-1.2
phage
P. simiae OR Antrim MOI 0.1
-0.0
N.D.
+0.2
-1.2
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.1
N.D.
+0.4
-0.3
-1.4
motility
outer cut, LB soft agar motility assay
+0.1
N.D.
+0.1
+0.1
-1.7
rhizosphere
rhizosphere sample 1; outgrowth in LB
-0.3
N.D.
+0.5
-0.7
-0.8
in planta
Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.2
N.D.
-0.1
-1.3
-0.2
seeds
Growth on radish seeds for 72 hours
+0.1
N.D.
+0.2
-0.8
-0.8
supernatant control
Vogels_fungal_media 0.1X
-0.4
N.D.
-0.1
-1.0
+0.2
supernatant control
Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB
+0.0
N.D.
+0.2
-1.2
-0.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.2
N.D.
+0.2
+0.2
-1.4
seeds
Growth on radish seeds for 72 hours
+0.4
N.D.
+0.1
-0.8
-1.0
soil
soil sample 5; outgrowth in LB
-0.1
N.D.
+0.3
-0.4
-0.8
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
-0.2
N.D.
+0.5
-0.5
-0.6
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
N.D.
-0.0
+0.5
-1.3
stress
R2A with Polymyxin B sulfate 0.003 mg/ml
-0.0
N.D.
+0.2
+0.6
-1.4
supernatant
Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x
-0.0
N.D.
+0.1
+0.7
-1.4
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days
+0.3
N.D.
+0.5
+0.0
-1.1
in planta
Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days
+0.1
N.D.
+0.5
+0.5
-1.3
stress
R2A with Polymyxin B sulfate 0.002 mg/ml
-0.1
N.D.
+0.2
+0.8
-1.0
phage
P. simiae OR1 MOI 0.1
+0.0
N.D.
-0.6
+1.4
-0.6
solid stress
Fraxetin 3 mM; solid stress
+0.5
N.D.
-0.7
+0.4
+0.3
in planta
Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days
-0.1
N.D.
-0.1
+3.1
-0.6
stress
Doxycycline hyclate 0.001 mg/ml
+0.5
N.D.
-0.2
+0.6
+1.8
stress
Gentamicin 0.008 mg/ml
-0.1
N.D.
+0.1
+0.4
+3.2
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