Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_24210 and PS417_24215 are separated by 15 nucleotidesPS417_24215 and PS417_24220 are separated by 170 nucleotidesPS417_24220 and PS417_24225 are separated by 3 nucleotidesPS417_24225 and PS417_24230 are separated by 178 nucleotides PS417_24210: PS417_24210 - dolichyl-phosphate-mannose-protein mannosyltransferase, at 5,250,649 to 5,252,082 _24210 PS417_24215: PS417_24215 - hypothetical protein, at 5,252,098 to 5,252,586 _24215 PS417_24220: PS417_24220 - hypothetical protein, at 5,252,757 to 5,254,577 _24220 PS417_24225: PS417_24225 - hypothetical protein, at 5,254,581 to 5,254,889 _24225 PS417_24230: PS417_24230 - ATP-dependent protease, at 5,255,068 to 5,257,506 _24230
Group Condition PS417_24210 PS417_24215 PS417_24220 PS417_24225 PS417_24230
stress methylglyoxal 0.032 vol% +0.2 N.D. -0.2 +0.4 -2.6
motility inner cut, LB soft agar motility assay -0.1 N.D. -0.0 -0.7 -1.0
supernatant control Vogels_fungal_media 0.1X -0.4 N.D. +0.0 -1.4 +0.1
motility outer cut, LB soft agar motility assay -0.0 N.D. -0.1 +0.0 -1.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 N.D. +0.2 -0.4 -1.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=inner; Collection=outgrowth_in_LBkan50 +0.0 N.D. +0.1 -0.8 -0.9
motility inner cut, LB soft agar motility assay -0.0 N.D. +0.3 -0.7 -1.2
phage P. simiae OR Antrim MOI 0.1 -0.0 N.D. +0.2 -1.2 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.1 N.D. +0.4 -0.3 -1.4
motility outer cut, LB soft agar motility assay +0.1 N.D. +0.1 +0.1 -1.7
rhizosphere rhizosphere sample 1; outgrowth in LB -0.3 N.D. +0.5 -0.7 -0.8
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 N.D. -0.1 -1.3 -0.2
seeds Growth on radish seeds for 72 hours +0.1 N.D. +0.2 -0.8 -0.8
supernatant control Vogels_fungal_media 0.1X -0.4 N.D. -0.1 -1.0 +0.2
supernatant control Vogels_fungal_media 0.2X; growth supplemented with 0.4X LB +0.0 N.D. +0.2 -1.2 -0.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.2 N.D. +0.2 +0.2 -1.4
seeds Growth on radish seeds for 72 hours +0.4 N.D. +0.1 -0.8 -1.0
soil soil sample 5; outgrowth in LB -0.1 N.D. +0.3 -0.4 -0.8
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days -0.2 N.D. +0.5 -0.5 -0.6
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 N.D. -0.0 +0.5 -1.3
stress R2A with Polymyxin B sulfate 0.003 mg/ml -0.0 N.D. +0.2 +0.6 -1.4
supernatant Supernatant; Trichoderma virens RS grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 0.25x -0.0 N.D. +0.1 +0.7 -1.4
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=20h_outgrowth_LB; Time=7_days +0.3 N.D. +0.5 +0.0 -1.1
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.1 N.D. +0.5 +0.5 -1.3
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.1 N.D. +0.2 +0.8 -1.0
phage P. simiae OR1 MOI 0.1 +0.0 N.D. -0.6 +1.4 -0.6
solid stress Fraxetin 3 mM; solid stress +0.5 N.D. -0.7 +0.4 +0.3
in planta Plant=Zea_mays; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16h_outgrowth_LB; Time=7_days -0.1 N.D. -0.1 +3.1 -0.6
stress Doxycycline hyclate 0.001 mg/ml +0.5 N.D. -0.2 +0.6 +1.8
stress Gentamicin 0.008 mg/ml -0.1 N.D. +0.1 +0.4 +3.2
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