Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_00230 and PS417_00235 are separated by 86 nucleotidesPS417_00235 and PS417_00240 overlap by 4 nucleotidesPS417_00240 and PS417_00245 are separated by 102 nucleotidesPS417_00245 and PS417_00250 overlap by 4 nucleotides PS417_00230: PS417_00230 - aminopeptidase, at 44,748 to 45,791 _00230 PS417_00235: PS417_00235 - HAD family hydrolase, at 45,878 to 46,537 _00235 PS417_00240: PS417_00240 - anhydrase, at 46,534 to 47,076 _00240 PS417_00245: PS417_00245 - oligopeptidase A, at 47,179 to 49,230 _00245 PS417_00250: PS417_00250 - DNA-binding protein, at 49,227 to 49,502 _00250
Group Condition PS417_00230 PS417_00235 PS417_00240 PS417_00245 PS417_00250
phage P. simiae ORA MOI 10 -0.6 -0.5 +0.0 -0.4 N.D.
agar plate interaction control Parafilmed volatile agar plate with no fungus -0.5 -0.5 -0.2 -0.2 N.D.
motility inner cut, LB soft agar motility assay -0.4 -0.2 -0.3 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 -0.5 +0.5 -1.0 N.D.
seeds Growth on radish seeds for 72 hours -0.1 +0.1 -0.5 -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.6 -0.2 +0.3 -0.5 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.2 -0.2 -0.8 N.D.
solid stress Fraxetin 3 mM; solid stress +0.2 -0.2 -0.3 -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.6 -0.3 -0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +0.2 -0.6 -0.2 -0.2 N.D.
agar plate interaction control Taped volatile agar plate with no fungus -0.4 +0.3 -0.1 -0.5 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -1.5 -0.4 -0.2 +1.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.3 +0.5 -0.1 -0.7 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Alanine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.2 +0.1 +0.7 -1.1 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.1 -0.4 +0.5 -0.7 N.D.
motility outer cut, LB soft agar motility assay +0.7 -0.1 -0.0 -0.8 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.0 +0.2 -0.8 N.D.
rhizosphere rhizosphere sample 1; outgrowth in LB +0.6 -0.6 -0.1 -0.0 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 -0.3 +0.4 -0.5 N.D.
phage P. simiae Grant Run MOI 10 +0.3 +0.3 -0.3 -0.4 N.D.
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.3 +0.4 +0.0 -0.6 N.D.
supernatant Supernatant; Trichoderma atroviride IMI dcr22mutant grown in Vogels_fungal_media, filtered; 0.8X -0.8 -0.2 +0.2 +0.9 N.D.
agar plate interaction Parafilmed volatile agar plate with Trichoderma atroviridae IMI dcr2mutant +0.9 -0.2 -0.0 -0.2 N.D.
stress R2A with Polymyxin B sulfate 0.002 mg/ml -0.4 +0.6 +0.2 +0.3 N.D.
stress Doxycycline hyclate 0.001 mg/ml +0.3 +0.8 -0.2 -0.0 N.D.
rhizosphere rhizosphere sample 8; outgrowth in LB +0.4 +0.2 +0.5 -0.2 N.D.
carbon source L-Valine (C) +0.2 +0.8 -0.3 +0.3 N.D.
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 +0.8 -0.2 -0.4 N.D.
stress R2A with Polymyxin B sulfate 0.003 mg/ml +0.6 +0.4 +0.2 +0.4 N.D.
in planta Plant=Zea_mays; PlantTreatment=25mM_NaCl; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.5 +0.4 +0.0 -0.2 N.D.
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