Fitness for 5 genes in Pseudomonas simiae WCS417

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500 ntPS417_02385 and PS417_02390 are separated by 45 nucleotidesPS417_02390 and PS417_02395 overlap by 1 nucleotidesPS417_02395 and PS417_02400 are separated by 73 nucleotidesPS417_02400 and PS417_02405 are separated by 173 nucleotides PS417_02385: PS417_02385 - esterase, at 544,811 to 545,419 _02385 PS417_02390: PS417_02390 - DNA topoisomerase IV subunit B, at 545,465 to 547,372 _02390 PS417_02395: PS417_02395 - DNA topoisomerase IV subunit B, at 547,372 to 548,376 _02395 PS417_02400: PS417_02400 - DNA topoisomerase IV subunit A, at 548,450 to 550,714 _02400 PS417_02405: PS417_02405 - hypothetical protein, at 550,888 to 551,601 _02405
Group Condition PS417_02385 PS417_02390 PS417_02395 PS417_02400 PS417_02405
agar plate interaction control Parafilmed volatile agar plate with no fungus -3.2 N.D. -0.5 N.D. -0.2
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Lysine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.5 N.D. -0.6 N.D. -0.5
phage P. simiae Grant Run MOI 0.1 -2.1 N.D. -0.4 N.D. -0.9
stress Phosphomycin 0.002 mg/ml -1.5 N.D. -0.5 N.D. -1.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Histidine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.9 N.D. -0.2 N.D. -1.1
stress methylglyoxal 0.032 vol% -2.0 N.D. -1.1 N.D. +0.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Proline; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -2.2 N.D. -0.6 N.D. +0.5
solid stress Fraxetin 2 mM; solid stress -1.8 N.D. -0.2 N.D. -0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Serine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -1.3 N.D. -0.8 N.D. -0.2
stress Doxycycline hyclate 0.001 mg/ml -1.8 N.D. -0.4 N.D. +0.3
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=Glycine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 -0.9 N.D. -1.1 N.D. +0.3
stress Chloramphenicol 0.0075 mg/ml -1.9 N.D. +0.1 N.D. +0.2
stress 1-ethyl-3-methylimidazolium acetate 35 mM -2.0 N.D. +0.5 N.D. -0.0
in planta Plant=Zea_mays-B73; PlantTreatment=None; Sample=rhizosphere; GrowthSubstrate=0.5X_MS_agar; Collection=outgrowth_LB; Time=7_days -1.9 N.D. +0.2 N.D. +0.5
stress Cisplatin 0.01 mg/ml +1.0 N.D. -1.0 N.D. -1.0
motility inner cut, LB soft agar motility assay +0.9 N.D. -1.2 N.D. -0.4
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Leucine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.9 N.D. -1.2 N.D. -0.2
motility outer cut, LB soft agar motility assay +0.7 N.D. -1.5 N.D. +0.3
solid stress 80% Methanol 3 mM; solid stress +0.9 N.D. -0.8 N.D. -0.5
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.2 N.D. -0.3 N.D. -1.1
motility_chemotaxis Chemotaxis:Motility:: Method=Plug_approach; Chemical=L-Arginine; Chemical_conc=5mM; Distance=2cm; Sample=outer; Collection=outgrowth_in_LBkan50 +0.7 N.D. -1.2 N.D. +0.3
phage P. simiae Grant Run MOI 10 +1.1 N.D. -0.4 N.D. -0.8
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.6 N.D. +0.0 N.D. -1.6
soil soil sample 3; outgrowth in LB +1.4 N.D. -1.3 N.D. +0.2
motility inner cut, LB soft agar motility assay +1.4 N.D. -1.3 N.D. +0.2
in planta Plant=Zea_mays; PlantTreatment=34_degrees_celsius; Sample=rhizosphere; GrowthSubstrate=sand_vermiculite_perlite_mix; Collection=16_outgrowth_LB; Time=7_days +1.3 N.D. -0.3 N.D. -0.7
stress R2A with Polymyxin B sulfate 0.003 mg/ml +1.2 N.D. +0.4 N.D. -1.1
stress R2A with Polymyxin B sulfate 0.002 mg/ml +1.4 N.D. +0.2 N.D. -1.1
stress R2A with Polymyxin B sulfate 0.003 mg/ml +2.1 N.D. +0.3 N.D. -0.8
stress Neomycin 0.04 mg/ml +3.1 N.D. +0.3 N.D. -0.4
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